The Airway Microbiota Signatures of Infection and Rejection in Lung Transplant Recipients/Experiment 3
From BugSigDB
Reviewed Marked as Reviewed by Peace Sandy on 2024-4-9
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Sputum Expectoration,Sputum,sputum
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Lung transplantation lung transplant,transplant of lung,transplantation of lung,Lung transplantation,lung transplantation
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Recipients with infection
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Recipients with rejection
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Lung transplant recipients (LTRs) with rejection
- Group 0 sample size Number of subjects in the control (unexposed) group
- 103
- Group 1 sample size Number of subjects in the case (exposed) group
- 31
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- None
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 4
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
Signature 1
Reviewed Marked as Reviewed by Peace Sandy on 2024-4-30
Source: Fig. 4C
Description: LEfSe analysis identifying the airway microbiota that were differentially altered among the transplant groups. Only those taxa with LDA scores 4.0 were ultimately considered.
Abundance in Group 1: increased abundance in Recipients with rejection
Revision editor(s): Scholastica
