Gut dysbiosis induces the development of depression-like behavior through abnormal synapse pruning in microglia-mediated by complement C3

From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-12-11
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Authors
Hao W, Ma Q, Wang L, Yuan N, Gan H, He L, Li X, Huang J, Chen J
Journal
Microbiome
Year
2024
Keywords:
Complement C3, Depression, Fecal microbiota transplantation, Gut microbiota, Microglia, Synaptic pruning
BACKGROUND: Remodeling eubiosis of the gut microenvironment may contribute to preventing the occurrence and development of depression. Mounting experimental evidence has shown that complement C3 signaling is associated with the pathogenesis of depression, and disruption of the gut microbiota may be an underlying cause of complement system activation. However, the mechanism by which complement C3 participates in gut-brain crosstalk in the pathogenesis of depression remains unknown. RESULTS: In the present study, we found that chronic unpredictable mild stress (CUMS)-induced mice exhibited obvious depression-like behavior as well as cognitive impairment, which was associated with significant gut dysbiosis, especially enrichment of Proteobacteria and elevation of microbiota-derived lipopolysaccharides (LPS). In addition, peripheral and central complement C3 activation and central C3/CR3-mediated aberrant synaptic pruning in microglia have also been observed. Transplantation of gut microbiota from CUMS-induced depression model mice into specific pathogen-free and germ-free mice induced depression-like behavior and concomitant cognitive impairment in the recipient mice, accompanied by increased activation of the complement C3/CR3 pathway in the prefrontal cortex and abnormalities in microglia-mediated synaptic pruning. Conversely, antidepressants and fecal microbiota transplantation from antidepressant-treated donors improved depression-like behaviors and restored gut microbiome disturbances in depressed mice. Concurrently, inhibition of the complement C3/CR3 pathway, amelioration of abnormal microglia-mediated synaptic pruning, and increased expression of the synapsin and postsynaptic density protein 95 were observed. Collectively, our results revealed that gut dysbiosis induces the development of depression-like behaviors through abnormal synapse pruning in microglia-mediated by complement C3, and the inhibition of abnormal synaptic pruning is the key to targeting microbes to treat depression. CONCLUSIONS: Our findings provide novel insights into the involvement of complement C3/CR3 signaling and aberrant synaptic pruning of chemotactic microglia in gut-brain crosstalk in the pathogenesis of depression. Video Abstract.

Experiment 1


Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine, Scholastica

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Response to antidepressant Response to antidepressant,response to antidepressant
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Chronic Unpredictable Mild Stress (CUMS), CUMS + Xiaoyaosan (XYS) and CUMS + fluoxetine (FLX) groups
Group 1 name Corresponds to the case (exposed) group for case-control studies
Control Group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Samples collected from the mice groups without any intervention or drug administration.
Group 0 sample size Number of subjects in the control (unexposed) group
18
Group 1 sample size Number of subjects in the case (exposed) group
6

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
4


Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 4F and Figure S2d

Description: LEfSe analysis of the microbiome data showing differentially abundant bacterial taxa in the control, CUMS, (CUMS + XYS) and (CUMS + FLX) groups.

Abundance in Group 1: increased abundance in Control Group

NCBI Quality ControlLinks
Acidimicrobiia
Actinomycetota
Armatimonadota
Bacillales
Bacillota
Chloroflexota
Deinococcota
Gemmatimonadota
Staphylococcaceae
Staphylococcus

Revision editor(s): Aleru Divine

Experiment 2


Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Control, (CUMS + XYS) and (CUMS + FLX) groups
Group 1 name Corresponds to the case (exposed) group for case-control studies
CUMS group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice group induced with chronic unpredictable mild stress (CUMS)

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 4F and Figure S2d

Description: LEfSe analysis of the microbiome data showing differentially abundant bacterial taxa in the control, CUMS, (CUMS + XYS) and (CUMS + FLX) groups.

Abundance in Group 1: increased abundance in CUMS group

NCBI Quality ControlLinks
Bacteroidaceae
Bacteroidales
Bacteroides
Bacteroidia
Enterobacterales
Enterobacteriaceae
Fusobacteriota
Gammaproteobacteria
Klebsiella
Pseudomonadota
Verrucomicrobiota

Revision editor(s): Aleru Divine

Experiment 3


Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Control, CUMS, and (CUMS + XYS) groups
Group 1 name Corresponds to the case (exposed) group for case-control studies
CUMS + FLX group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice group induced with chronic unpredictable mild stress (CUMS) for 8 weeks, followed by daily oral administration of fluoxetine (FLX).

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 4F and Figure S2d

Description: LEfSe analysis of the microbiome data showing differentially abundant bacterial taxa in the control, CUMS, (CUMS + XYS) and (CUMS + FLX) groups.

Abundance in Group 1: increased abundance in CUMS + FLX group

NCBI Quality ControlLinks
Acidobacteriota
Allobaculum
Atribacterota
Bacteroidota
Chlorobiota
Cyanobacteriota
Deferribacterota
Erysipelotrichaceae
Erysipelotrichales
Erysipelotrichia
Planctomycetota

Revision editor(s): Aleru Divine

Experiment 4


Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Control, CUMS, and (CUMS + FLX) groups
Group 1 name Corresponds to the case (exposed) group for case-control studies
CUMS + XYS group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice group induced with chronic unpredictable mild stress (CUMS) for 8 weeks, followed by daily oral administration of Xiaoyaosan (XYS).

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 4F and Figure S2d

Description: LEfSe analysis of the microbiome data showing differentially abundant bacterial taxa in the control, CUMS, (CUMS + XYS) and (CUMS + FLX) groups.

Abundance in Group 1: increased abundance in CUMS + XYS group

NCBI Quality ControlLinks
Bacilli
Bacillota
Lactobacillaceae
Lactobacillales
Lactobacillus
Mycoplasmatota

Revision editor(s): Aleru Divine

Experiment 5


Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Environmental stress treatment environmental stress,EnvironmentalStress,Environmental stress treatment,environmental stress treatment
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Control group
Group 1 name Corresponds to the case (exposed) group for case-control studies
CUMS Group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice group induced with chronic unpredictable mild stress (CUMS)
Group 0 sample size Number of subjects in the control (unexposed) group
6

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 8g

Description: LEfSe analysis of the microbiome data showing differentially abundant bacterial taxa in the control and CUMS group

Abundance in Group 1: increased abundance in CUMS Group

NCBI Quality ControlLinks
Prevotella
unclassified Paraprevotella

Revision editor(s): Aleru Divine

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 8G

Description: LEfSe analysis of the microbiome data showing differentially abundant bacterial taxa in the control and CUMS group

Abundance in Group 1: decreased abundance in CUMS Group

NCBI Quality ControlLinks
Bacteria
Faecalibacterium

Revision editor(s): Aleru Divine

Experiment 6


Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Response to transplant Response to transplant,response to transplant
Group 1 name Corresponds to the case (exposed) group for case-control studies
Control + FMT(CUMS) group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice group that received fecal microbiota transplantation from host induced with chronic unpredictable mild stress (CUMS).

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 8i

Description: LEfSe analysis of the microbiome data showing differentially abundant bacterial taxa in the control and Control + FMT(CUMS) group.

Abundance in Group 1: increased abundance in Control + FMT(CUMS) group

NCBI Quality ControlLinks
Allobaculum
Bacteroidales
Bacteroidia
Bacteroidota
Erysipelotrichaceae
Erysipelotrichales
Erysipelotrichia
Parabacteroides
Porphyromonadaceae
Prevotella
unclassified Paraprevotella

Revision editor(s): Aleru Divine

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 8i

Description: LEfSe analysis of the microbiome data showing differentially abundant bacterial taxa in the control and Control + FMT(CUMS) group.

Abundance in Group 1: decreased abundance in Control + FMT(CUMS) group

NCBI Quality ControlLinks
Bacillota

Revision editor(s): Aleru Divine

Experiment 7


Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
CUMS group
Group 1 name Corresponds to the case (exposed) group for case-control studies
CUMS + FMT(XYS) group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice group that received fecal microbiota transplantation from host treated with Xiaoyaosan (XYS).

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 8K

Description: LEfSe analysis of the microbiome data showing differentially abundant bacterial taxa in the control and CUMS + FMT(XYS) group.

Abundance in Group 1: increased abundance in CUMS + FMT(XYS) group

NCBI Quality ControlLinks
Bacteria
Prevotella
unclassified Paraprevotella

Revision editor(s): Aleru Divine

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/03/22

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 8K

Description: LEfSe analysis of the microbiome data showing differentially abundant bacterial taxa in the control and CUMS + FMT(XYS) group.

Abundance in Group 1: decreased abundance in CUMS + FMT(XYS) group

NCBI Quality ControlLinks
Candidatus Arthromitus
Dialister
Ruminococcus

Revision editor(s): Aleru Divine

Experiment 8


Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/11/07

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Control group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Control + FMT(CUMS) group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice group that received fecal microbiota transplantation (FMT) from host induced with chronic unpredictable mild stress (CUMS).

Lab analysis

Statistical Analysis

Statistical test
Pearson Correlation
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
Not specified

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/11/07

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure S5F

Description: Gut microbiota change at genus level (Control versus Control+FMT(CUMS))

Abundance in Group 1: increased abundance in Control + FMT(CUMS) group

NCBI Quality ControlLinks
Allobaculum
Prevotella
Parabacteroides
Sutterella
Bacteroides

Revision editor(s): Aleru Divine

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/11/07

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure S5F

Description: Gut microbiota change at genus level (Control versus Control+FMT(CUMS))

Abundance in Group 1: decreased abundance in Control + FMT(CUMS) group

NCBI Quality ControlLinks
Gardnerella
Clostridium
Staphylococcus
Roseburia
Candidatus Arthromitus
Lactobacillus
Coprococcus
Oscillospira
Ruminococcus
Helicobacter
Faecalibacterium
unidentified
Blautia
Prevotella
Akkermansia

Revision editor(s): Aleru Divine

Experiment 9


Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/11/07

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
CUMS group
Group 1 name Corresponds to the case (exposed) group for case-control studies
CUMS + FMT(XYS) group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice group that received fecal microbiota transplantation from host treated with Xiaoyaosan (XYS).

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/11/07

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure S5d

Description: Gut microbiota change at genus level (CUMS versus CUMS+FMT(XYS)).

Abundance in Group 1: increased abundance in CUMS + FMT(XYS) group

NCBI Quality ControlLinks
Akkermansia
Lactobacillus
Sutterella
Prevotella
Parabacteroides
Bacteroides

Revision editor(s): Aleru Divine

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/11/07

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure S5d

Description: Gut microbiota change at genus level (CUMS versus CUMS+FMT(XYS)).

Abundance in Group 1: decreased abundance in CUMS + FMT(XYS) group

NCBI Quality ControlLinks
Faecalibacterium
Clostridium
Gardnerella
Allobaculum
unidentified
Roseburia
Helicobacter
Coprococcus
Blautia
Ruminococcus
Prevotella
Oscillospira
Candidatus Arthromitus
Staphylococcus

Revision editor(s): Aleru Divine

Experiment 10


Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/11/07

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Environmental stress treatment environmental stress,EnvironmentalStress,Environmental stress treatment,environmental stress treatment
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Control group
Group 1 name Corresponds to the case (exposed) group for case-control studies
CUMS Group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice group induced with chronic unpredictable mild stress (CUMS)

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/11/07

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure S5E

Description: Gut microbiota change at genus level (Control versus CUMS).

Abundance in Group 1: increased abundance in CUMS Group

NCBI Quality ControlLinks
Allobaculum
Oscillospira
Clostridium
Prevotella
Gardnerella
Parabacteroides
Helicobacter
Akkermansia
Prevotella sp.

Revision editor(s): Aleru Divine

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/11/07

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure S5E

Description: Gut microbiota change at genus level (Control versus CUMS).

Abundance in Group 1: decreased abundance in CUMS Group

NCBI Quality ControlLinks
Candidatus Arthromitus
Bacteroides
Lactobacillus
Ruminococcus
Staphylococcus
Coprococcus
unidentified
Sutterella
Roseburia
Faecalibacterium
Blautia

Revision editor(s): Aleru Divine

Experiment 11


Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/12/11

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Depressive disorder depression,melancholia,melancholias,mental depression,syndrome, depressive,syndromes, depressive,Depressive disorder,depressive disorder
Group 1 name Corresponds to the case (exposed) group for case-control studies
CUMS group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice group induced with chronic unpredictable mild stress (CUMS) for 8 weeks.

Lab analysis

Statistical Analysis

Statistical test
Kruskall-Wallis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/12/11

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 4(G-I)

Description: The relative abundances of selected bacterial signatures in the control, CUMS, (CUMS + XYS) and (CUMS + FLX) groups.

Abundance in Group 1: increased abundance in CUMS group

NCBI Quality ControlLinks
Bacteroides
Klebsiella

Revision editor(s): Aleru Divine

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/12/11

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 4(G-I)

Description: The relative abundances of selected bacterial signatures in the control, CUMS, (CUMS + XYS) and (CUMS + FLX) groups.

Abundance in Group 1: decreased abundance in CUMS group

NCBI Quality ControlLinks
Lactobacillus

Revision editor(s): Aleru Divine

Experiment 12


Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/12/11

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Response to antidepressant Response to antidepressant,response to antidepressant
Group 0 name Corresponds to the control (unexposed) group for case-control studies
CUMS group
Group 1 name Corresponds to the case (exposed) group for case-control studies
CUMS + XYS group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice group induced with chronic unpredictable mild stress (CUMS) for 8 weeks, followed by daily oral administration of Xiaoyaosan (XYS).

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/12/11

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 4(G-I)

Description: The relative abundances of selected bacterial signatures in the control, CUMS, (CUMS + XYS) and (CUMS + FLX) groups.

Abundance in Group 1: increased abundance in CUMS + XYS group

NCBI Quality ControlLinks
Lactobacillus

Revision editor(s): Aleru Divine

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/12/11

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 4(G-I)

Description: The relative abundances of selected bacterial signatures in the control, CUMS, (CUMS + XYS) and (CUMS + FLX) groups.

Abundance in Group 1: decreased abundance in CUMS + XYS group

NCBI Quality ControlLinks
Bacteroides
Klebsiella

Revision editor(s): Aleru Divine

Experiment 13


Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/12/11

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
CUMS + FLX group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice group induced with chronic unpredictable mild stress (CUMS) for 8 weeks, followed by daily oral administration of fluoxetine (FLX).

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-12-11

Curated date: 2024/12/11

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 4(G-I)

Description: The relative abundances of selected bacterial signatures in the control, CUMS, (CUMS + XYS) and (CUMS + FLX) groups.

Abundance in Group 1: decreased abundance in CUMS + FLX group

NCBI Quality ControlLinks
Bacteroides
Klebsiella

Revision editor(s): Aleru Divine