Comparison of fecal and oral collection methods for studies of the human microbiota in two Iranian cohorts/Experiment 2
From BugSigDB
Subjects
- Location of subjects
- Iran
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- OMNIgene ORAL kits
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Scope mouthwash
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Oral sample collected with Scope mouthwash
- Group 0 sample size Number of subjects in the control (unexposed) group
- 77
- Group 1 sample size Number of subjects in the case (exposed) group
- 125
Lab analysis
- Sequencing type
- PCR
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Spearman Correlation
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Matched on Factors on which subjects have been matched on in a case-control study
- age, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Source: Additional files 2 Table S8
Description: Differential abundance analysis of phylum level changes comparing OMNIgene ORAL kit samples to Scope mouthwash oral samples from Gonbad and Yazd, Iran
Abundance in Group 1: increased abundance in Scope mouthwash
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidota | ||
| Campylobacterota | ||
| Patescibacteria group | ||
| Spirochaetota |
Revision editor(s): EGO
Signature 2
Source: Additional files 2 Table S8
Description: Differential abundance analysis of phylum level changes comparing OMNIgene ORAL kit samples to Scope mouthwash oral samples from Gonbad and Yazd, Iran
Abundance in Group 1: decreased abundance in Scope mouthwash
| NCBI | Quality Control | Links |
|---|---|---|
| Pseudomonadota |
Revision editor(s): EGO
