Gut microbiome profiling of a rural and urban South African cohort reveals biomarkers of a population in lifestyle transition/Experiment 1
From BugSigDB
Subjects
- Location of subjects
- South Africa
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Lifestyle measurement Lifestyle measurement,lifestyle measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Female subjects situated in Bushbuckridge
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Female subjects situated in Soweto
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Female subjects (both obese and lean) living in Soweto whose microbiota was studied to reflect transitional changes in microbiome on account of the adoption of a more Westernized lifestyle (in terms of diet and activity levels). Soweto represented the urban site of the two; the other (Bushbuckridge) being more relatively rural.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 119
- Group 1 sample size Number of subjects in the case (exposed) group
- 51
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- HIV infection, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
Signature 1
Source: Figure 5a and Supplementary Table 2A
Description: Phylum and Genus level significant differential abundance of taxa between Bushbuckbridge and Soweto cohorts (site differences).
Abundance in Group 1: increased abundance in Female subjects situated in Soweto
| NCBI | Quality Control | Links |
|---|---|---|
| Acetanaerobacterium | ||
| Streptococcus | ||
| Bacillota |
Signature 2
Source: Figure 5a and Supplementary Table 2A
Description: Phylum and Genus level significant differential abundance of taxa between Bushbuckbridge and Soweto cohorts (site differences).
Abundance in Group 1: decreased abundance in Female subjects situated in Soweto
