Changes in the vaginal microbiota associated with primary ovarian failure

From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-6-17
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Wang J, Xu J, Han Q, Chu W, Lu G, Chan WY, Qin Y, Du Y
Journal
BMC microbiology
Year
2020
Keywords:
16S rRNA, Female reproductive tract, Pathogenesis, Primary ovarian failure, Vaginal microbiota
BACKGROUND: Primary ovarian failure (POF) is defined as follicular failure in women of reproductive age. Although many factors are speculated to contribute to the occurrence of POF, the exact aetiology remains unclear. Moreover, alterations in the microbiome of patients with POF are poorly studied. RESULTS: This study investigated the vaginal microbiota of 22 patients with POF and 29 healthy individuals. High-throughput Illumina MiSeq sequencing targeting the V3-V4 region of the 16S ribosomal RNA (rRNA) gene was used to evaluate the relationships between the vaginal flora and clinical characteristics of POF. Different from results of previous studies, we found that the diversity and richness of the vaginal flora of patients with POF was significantly different from those of healthy controls. Comparison of the vaginal flora of patients with POF with that of menopausal women revealed that the relative abundance of Lactobacillus was significantly reduced in the latter. A reduced abundance of Lactobacillus was furthermore associated with a lower pregnancy success rate. Of particular interest is that L. gallinarum especially appeared to be beneficially associated with reproductive-related indicators (FSH, E2, AMH, PRL) whilst L. iners appeared to have a detrimental effect. The result of the present study may enable the identification of microbiota associated with POF, however, further investigations of differences in the microbiota in the context of POF will enable a deeper understanding of the disease pathogenesis that involves modification of the vaginal microbiota. CONCLUSIONS: The present study identified the microbiota associated with POF. Further investigations on the differences in the microbiota in the context of POF will improve our understanding of the pathogenesis of the disease which involves modification of the vaginal microbiota.

Experiment 1


Reviewed Marked as Reviewed by Svetlana up on 2024-6-17

Curated date: 2024/03/25

Curator: Ndruscilla

Revision editor(s): Ndruscilla, Scholastica

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Vaginal fluid Vaginal discharge,Vaginal secretion,Vaginal fluid,vaginal fluid
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Acquired primary ovarian failure acquired premature ovarian failure,acquired primary ovarian failure,Acquired primary ovarian failure
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy controls
Group 1 name Corresponds to the case (exposed) group for case-control studies
Primary ovarian failure (POF)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Women of reproductive age with follicular failure
Group 0 sample size Number of subjects in the control (unexposed) group
29
Group 1 sample size Number of subjects in the case (exposed) group
22
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
3 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Chao1 Abundance-based estimator of species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased
Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-6-17

Curated date: 2024/03/25

Curator: Ndruscilla

Revision editor(s): Ndruscilla, Scholastica

Source: Figure 2a

Description: Differential genera in vaginal microbiota in patients with primary ovarian failure (POF) compared to control group

Abundance in Group 1: increased abundance in Primary ovarian failure (POF)

NCBI Quality ControlLinks
Prevotella
Gardnerella
Bifidobacterium
Escherichia/Shigella sp.
Bacteroides
Campylobacter
Peptoniphilus
Anaerococcus
Streptococcus
Helcococcus
Providencia
Corynebacterium
Clostridium XIVaClostridium XIVa
Arcobacter
Oceanobacillus
Chryseobacterium
Finegoldia
Clostridium XIClostridium XI
Howardella
Blautia
Luteimonas
Caryophanon
Ruminococcus2Ruminococcus2
Marinobacter
Murdochiella
Gemella
Gemmiger
Rothia
Arcanobacterium

Revision editor(s): Ndruscilla, Scholastica

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-6-17

Curated date: 2024/03/25

Curator: Ndruscilla

Revision editor(s): Ndruscilla, Scholastica

Source: Figure 2a

Description: Differential genera in vaginal microbiota in patients with primary ovarian failure (POF) compared to control group

Abundance in Group 1: decreased abundance in Primary ovarian failure (POF)

NCBI Quality ControlLinks
Lactobacillus
Brevundimonas
Parvimonas
Roseburia
Ochrobactrum
Atopobium

Revision editor(s): Ndruscilla, Scholastica