The Intestinal Microbiota in Acute Anorexia Nervosa and During Renourishment: Relationship to Depression, Anxiety, and Eating Disorder Psychopathology

Study information

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-10

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

26428446

DOI Digital object identifier for electronic documents.

10.1097/PSY.0000000000000247

URI

Authors

Kleiman SC, Watson HJ, Bulik-Sullivan EC, Huh EY, Tarantino LM, Bulik CM, Carroll IM

Journal

Psychosomatic medicine

Year

2015

OBJECTIVE: The relevance of the microbe-gut-brain axis to psychopathology is of interest in anorexia nervosa (AN), as the intestinal microbiota plays a critical role in metabolic function and weight regulation. METHODS: We characterized the composition and diversity of the intestinal microbiota in AN, using stool samples collected at inpatient admission (T1; n = 16) and discharge (T2; n = 10). At T1, participants completed the Beck Depression and Anxiety Inventories and the Eating Disorder Examination-Questionnaire. Patients with AN were compared with healthy individuals who participated in a previous study (healthy comparison group; HCG). Genomic DNA was isolated from stool samples, and bacterial composition was characterized by 454 pyrosequencing of the 16S rRNA gene. Sequencing results were processed by the Quantitative Insights Into Microbial Ecology pipeline. We compared T1 versus T2 samples, samples from both points were compared with HCG (n = 12), and associations between psychopathology and T1 samples were explored. RESULTS: In patients with AN, significant changes emerged between T1 and T2 in taxa abundance and beta (between-sample) diversity. Patients with AN had significantly lower alpha (within-sample) diversity than did HCG at both T1 (p = .0001) and T2 (p = .016), and differences in taxa abundance were found between AN patients and HCG. Levels of depression, anxiety, and eating disorder psychopathology at T1 were associated with composition and diversity of the intestinal microbiota. CONCLUSIONS: We provide evidence of an intestinal dysbiosis in AN and an association between mood and the enteric microbiota in this patient population. Future directions include mechanistic investigations of the microbe-gut-brain axis in animal models and association of microbial measures with metabolic changes and recovery indices.

Experiment 1

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Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-10

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Subjects

Location of subjects: United States of America

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Anorexia nervosa anorexia nervosa,Anorexia nervosa

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy comparison group (HCG)

Group 1 name Corresponds to the case (exposed) group for case-control studies: Anorexia patients at T1

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Patients with anorexia at hospital admission (T1)

Group 0 sample size Number of subjects in the control (unexposed) group: 12

Group 1 sample size Number of subjects in the case (exposed) group: 16

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 2 months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V1-V3

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Roche454

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Matched on Factors on which subjects have been matched on in a case-control study: age, sex

Alpha Diversity

Chao1 Abundance-based estimator of species richness: decreased

Richness Number of species: decreased

Signature 1

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Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-10

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Table 2

Description: Differences in microbial taxa among anorexia patients at hospital admission (T1) VS healthy comparison group (HCG)

Abundance in Group 1: increased abundance in Anorexia patients at T1

NCBI	Quality Control	Links
Bacilli
Coriobacteriales

Revision editor(s): WikiWorks, ChiomaBlessing

Signature 2

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Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-10

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Table 2

Description: Differences in microbial taxa among anorexia patients at hospital admission (T1) VS healthy comparison group (HCG)

Abundance in Group 1: decreased abundance in Anorexia patients at T1

NCBI	Quality Control	Links
Anaerostipes
Clostridia
Eubacteriales
Faecalibacterium

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 2

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Curated date: 2021/01/10

Curator: WikiWorks

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Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies: Anorexia patients at T2

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Patients with anorexia at hospital discharge (T2)