Dysbiosis of gut microbiota and its correlation with dysregulation of cytokines in psoriasis patients

Study information

Reviewed Marked as Reviewed by Svetlana up on 2024-6-17

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

33685393

DOI Digital object identifier for electronic documents.

10.1186/s12866-021-02125-1

URI

https://rdcu.be/dCvoU

Authors

Zhang X, Shi L, Sun T, Guo K, Geng S

Journal

BMC microbiology

Year

2021

Keywords:

16S, Cytokines, Gut microbiome, Microbiota, Psoriasis

BACKGROUND: Psoriasis is an inflammatory skin disease associated with multiple comorbidities and substantially diminishes patients' quality of life. The gut microbiome has become a hot topic in psoriasis as it has been shown to affect both allergy and autoimmunity diseases in recent studies. Our objective was to identify differences in the fecal microbial composition of patients with psoriasis compared with healthy individuals to unravel the microbiota profiling in this autoimmune disease. RESULTS: We collected fecal samples from 30 psoriasis patients and 30 healthy controls, sequenced them by 16S rRNA high-throughput sequencing, and identified the gut microbial composition using bioinformatic analyses including Quantitative Insights into Microbial Ecology (QIIME) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Our results showed that different relative abundance of certain bacterial taxa between psoriasis patients and healthy individuals, including Faecalibacterium and Megamonas, were increased in patients with psoriasis. It's also implicated that many cytokines act as main effect molecules in the pathology of psoriasis. We selected the inflammation-related indicators that were abnormal in psoriasis patients and found the microbiome variations were associated with the level of them, especially interleukin-2 receptor showed a positive relationship with Phascolarctobacterium and a negative relationship with the Dialister. The relative abundance of Phascolarctobacterium and Dialister can be regard as predictors of psoriasis activity. The correlation analysis based on microbiota and Inflammation-related indicators showed that microbiota dysbiosis might induce an abnormal immune response in psoriasis. CONCLUSIONS: We concluded that the gut microbiome composition in psoriasis patients has been altered markedly and provides evidence to understand the relationship between gut microbiota and psoriasis. More mechanistic experiments are needed to determine whether the differences observed in gut microbiota are the cause or consequences of psoriasis and whether the relationship between gut microbiota and cytokines was involved.

Experiment 1

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Reviewed Marked as Reviewed by Svetlana up on 2024-6-17

Curated date: 2024/03/26

Curator: PraiseAgbetuyi

Revision editor(s): PraiseAgbetuyi, KwennB, Scholastica

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Psoriasis Other and unspecified pityriasis,OTHER PSORIASIS,Other psoriasis and similar disorders,Other psoriasis and similar disorders (disorder),Other psoriasis and similar disorders excluding psoriatic arthropathy,Palmoplantaris Pustulosis,PITYRIASIS NEC & NOS,PSORIAS RELATED DIS NEC,Psoriases,psoriasis,Psoriasis and similar disorders,Psoriasis and similar disorders (disorder),Psoriasis and similar disorders (navigational concept),Psoriasis and similar disorders NOS,Psoriasis and similar disorders NOS (disorder),Pustular Psoriasis of Palms and Soles,PUSTULAR PSORIASIS OF PALMS SOLES,Pustulosis of Palms and Soles,PUSTULOSIS OF PALMS SOLES,Pustulosis Palmaris et Plantaris,Psoriasis

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy Control (HC)

Group 1 name Corresponds to the case (exposed) group for case-control studies: Psoriasis

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Patients with a clinical diagnosis of psoriasis who were attending a dermatology outpatient clinic in the Second Affiliated Hospital of Xi ‘an Jiaotong University, among them 24 were diagnosed with psoriasis vulgaris (PV), 6 with pustular psoriasis (PP).

Group 0 sample size Number of subjects in the control (unexposed) group: 30

Group 1 sample size Number of subjects in the case (exposed) group: 30

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 1 month

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2.00

Matched on Factors on which subjects have been matched on in a case-control study: age, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Richness Number of species: unchanged

Signature 1

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Curated date: 2024/03/28

Curator: PraiseAgbetuyi

Revision editor(s): PraiseAgbetuyi

Source: Figure 2 e/f

Description: Differentially abundant taxa between the psoriasis group (P) and healthy control group (N) which was generated from LEfSe analysis

Abundance in Group 1: increased abundance in Psoriasis

NCBI	Quality Control	Links
Faecalibacterium
Gemmiger
Megamonas
Pyramidobacter
Synergistales
Synergistes
Synergistia
Veillonellaceae
Dethiosulfovibrionaceae

Revision editor(s): PraiseAgbetuyi

Signature 2

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Curated date: 2024/03/28

Curator: PraiseAgbetuyi

Revision editor(s): PraiseAgbetuyi

Source: Figure 2 e/f

Description: Differentially abundant taxa between the psoriasis group (P) and healthy control group (N) which was generated from LEfSe analysis

Abundance in Group 1: decreased abundance in Psoriasis

NCBI	Quality Control	Links
Fusobacterium
Fusobacteriaceae
Bacteroidaceae
Bacteroides

Revision editor(s): PraiseAgbetuyi

Experiment 2

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Reviewed Marked as Reviewed by Svetlana up on 2024-6-17

Curated date: 2024/03/29

Curator: KwennB

Revision editor(s): KwennB, Scholastica

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Gut microbiome measurement Gut microbiome measurement,gut microbiome measurement

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Pustular Psoriasis (PP)

Group 1 name Corresponds to the case (exposed) group for case-control studies: Psoriasis Vulgaris (PV)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Patients with psoriasis diagnosed with psoriasis vulgaris (PV)