Bald sea urchin disease shifts the surface microbiome on purple sea urchins in an aquarium/Experiment 6

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Reviewed Marked as Reviewed by Svetlana up on 2025-1-22

Curated date: 2024/04/16

Curator: Omojokunoluwatomisin

Revision editor(s): Omojokunoluwatomisin, KateRasheed

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Strongylocentrotus purpuratus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Anatomical surface Anatomical surface,anatomical surface
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Bacterial disease Bacteria caused disease or disorder,Bacteria disease or disorder,Bacteria infectious disease,bacterial disease,bacterial disorder,bacterial infection,bacterial infectious disease,infection, bacterial,infections, bacterial,Bacterial disease
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Recovered + Healthy sea urchins - ZymoResearch pipeline
Group 1 name Corresponds to the case (exposed) group for case-control studies
Diseased sea urchins - ZymoResearch pipeline
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Bald sea urchin disease (BSUD) is a bacterial infection that occurs in a wide range of sea urchin species and causes the loss of surface appendages.
Group 0 sample size Number of subjects in the control (unexposed) group
8
Group 1 sample size Number of subjects in the case (exposed) group
4

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2


Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-1-22

Curated date: 2024/04/17

Curator: Omojokunoluwatomisin

Revision editor(s): Omojokunoluwatomisin, KateRasheed

Source: Supplementary Data file 3, TableS4-5.

Description: Taxa have different abundances in microbiomes from samples collected from diseased sea urchins compared to recovered and healthy sea urchins

Abundance in Group 1: increased abundance in Diseased sea urchins - ZymoResearch pipeline

NCBI Quality ControlLinks
Arcobacter
Blastopirellula
Colwellia
Erwinia
Erwinia rhapontici
Fluviicola
Leucothrix
Lutibacter
Lutibacter agarilyticus
Octadecabacter
Polaribacter
unclassified Oceanospirillaceae
Leucothrix mucor sp64100Leucothrix mucor sp64100
Blastopirellula sp41404-sp41424Blastopirellula sp41404-sp41424
Arcobacter sp55236Arcobacter sp55236
Fluviicola sp.
Colwellia meonggei sp56501Colwellia meonggei sp56501
Colwellia meonggei sp56501-sp56505Colwellia meonggei sp56501-sp56505
unclassified Pseudobdellovibrionaceae
unclassified Saprospiraceae

Revision editor(s): Omojokunoluwatomisin, KateRasheed