Bald sea urchin disease shifts the surface microbiome on purple sea urchins in an aquarium/Experiment 7

From BugSigDB


Needs review

Curated date: 2024/04/17

Curator: Omojokunoluwatomisin

Revision editor(s): Omojokunoluwatomisin, Carita Ndibe

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Strongylocentrotus purpuratus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Anatomical surface Anatomical surface,anatomical surface
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Bacterial disease Bacteria caused disease or disorder,Bacteria disease or disorder,Bacteria infectious disease,bacterial disease,bacterial disorder,bacterial infection,bacterial infectious disease,infection, bacterial,infections, bacterial,Bacterial disease
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Sea water from recovered sea urchins aquarium
Group 1 name Corresponds to the case (exposed) group for case-control studies
Sea water diseased sea urchins aquarium
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
The sample collected from the water in the aquarium where the sea urchins with Bald sea urchin disease (BSUD) were cultured.
Group 0 sample size Number of subjects in the control (unexposed) group
2
Group 1 sample size Number of subjects in the case (exposed) group
2

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina, Non-quantitative PCR

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
3

Alpha Diversity

Chao1 Abundance-based estimator of species richness
decreased

Signature 1

Needs review

Curated date: 2024/04/23

Curator: Carita Ndibe

Revision editor(s): Carita Ndibe

Source: Supplementary Data File 2, Table S6

Description: Relative abundance of the most abundant genera in the seawater samples

Abundance in Group 1: increased abundance in Sea water diseased sea urchins aquarium

NCBI Quality ControlLinks
Vibrio
Colwellia
RhodobacteraceaeRhodobacteraceae
Leucothrix
NCBI:txid97050NCBI:txid97050
Erwiniaceae
Lutibacter
NS10 marine groupNS10 marine group

Revision editor(s): Carita Ndibe

Signature 2

Needs review

Curated date: 2024/04/23

Curator: Carita Ndibe

Revision editor(s): Carita Ndibe

Source: Supplementary Data File 2, Table S6

Description: Relative abundance of the most abundant genera in the seawater samples

Abundance in Group 1: decreased abundance in Sea water diseased sea urchins aquarium

NCBI Quality ControlLinks
Alphaproteobacteria
Alteromonadaceae
Bacteroidia
Desulfotalea
Gammaproteobacteria
Marinifilaceae
Pseudahrensia
Pseudoalteromonas
Psychromonas
Roseimarinus
Spongiibacteraceae
Bacteroidetes BD2-2Bacteroidetes BD2-2
vadinHA21vadinHA21

Revision editor(s): Carita Ndibe