Gut microbiota: effect of pubertal status

Study information

Needs review

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

33143658

DOI Digital object identifier for electronic documents.

https://doi.org/10.1186/s12866-020-02021-0

URI

Authors

Yuan X, Chen R, Zhang Y, Lin X, Yang X

Journal

BMC microbiology

Year

2020

Keywords:

16 s rRNA, Adolescent, Children, Gut microbiota, Puberty

BACKGROUND: The make-up of gut microbiota at different puberty stages has not been reported. This cross-sectional study analyzed the bio-diversity of gut microbiota at different puberty stages. RESULT: The subjects (aged 5-15 years) were divided into non-pubertal (n = 42, male%: 66.7%) or pubertal groups (n = 47, male%:44.68); in both groups, Firmicutes, Bacteroidetes and Proteobacteria were the dominant phylum. There was no difference of alpha- and beta-diversity among disparate puberty stages. Non-pubertal subjects had members of the order Clostridiales, family Clostridiaceae, genus Coprobacillus which were significantly more prevalent than puberty subjects. Also, the pubertal subjects had members of class Betaproteobacteria, order Burkholderiales which were significantly more prevalent than the non-pubertal subjects. Their relative abundance was independent of BMI-Z. In the pubertal subjects, the abundance of genus Adlercreutzia, Ruminococcus, Dorea, Clostridium and Parabacteroides was associated with the level of testosterone. CONCLUSIONS: This is the first report of the diversity of gut microbiota at different puberty stages. The various species of gut microbiota changed gradually associated with puberty stages. Differences in gut microflora at different pubertal status may be related to androgen levels.

Experiment 1

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Curated date: 2024/03/27

Curator: Junie

Revision editor(s): Junie, Nityasinghal 14

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Puberty Puberty,puberty

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Non-pubertal

Group 1 name Corresponds to the case (exposed) group for case-control studies: Pubertal

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Individuals who have undergone puberty.

Group 0 sample size Number of subjects in the control (unexposed) group: 42

Group 1 sample size Number of subjects in the case (exposed) group: 47

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Alpha Diversity

Pielou Quantifies how equal the community is numerically: unchanged

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample: unchanged

Signature 1

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Curated date: 2024/03/28

Curator: Nityasinghal 14

Revision editor(s): Nityasinghal 14, Junie

Source: Figure 5

Description: Differential biomarkers associated with genders in pubertal subjects and non-pubertal subjects.

Abundance in Group 1: increased abundance in Pubertal

NCBI	Quality Control	Links
Betaproteobacteria
Burkholderiales

Revision editor(s): Nityasinghal 14, Junie

Signature 2

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Mark reviewed

Your review change was submitted

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Curated date: 2024/03/28

Curator: Nityasinghal 14

Revision editor(s): Nityasinghal 14, Junie

Source: Figure 5

Description: Differential biomarkers associated with genders in pubertal subjects and non-pubertal subjects.

Abundance in Group 1: decreased abundance in Pubertal

NCBI	Quality Control	Links
Pasteurellales
Clostridiaceae
Haemophilus
Coprobacillus
Eubacteriales

Revision editor(s): Nityasinghal 14, Junie