Stachyose ameliorates obesity-related metabolic syndrome via improving intestinal barrier function and remodeling gut microbiota

Study information

Reviewed Marked as Reviewed by Svetlana up on 2024-6-9

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

DOI Digital object identifier for electronic documents.

10.1016/j.jff.2024.106106

URI

https://www.sciencedirect.com/science/article/pii/S1756464624001087#s0125

Authors

Haoyu Wang, Kaiwei Chen, Liang Xiao, Ningning He, Shangyong Li, Shengnan Yu, Xiaoqian Lin, Yuanqiang Zou, Zhinan Wu

Journal

Journal of Functional Foods

Year

2024

Pages:

12

First page:

1

Keywords:

Butyrate-producing strains, Stachyose, gut microbiota, intestinal barrier, metabolic syndrome

In this study, oral supplementation of stachyose significantly improved HFD-induced MetS symptom, including overweight, hyperlipidemia, hepatic steatosis and system-wide inflammation. The subsequent analysis revealed that stachyose supplement significantly enhanced the integrity of the intestinal epithelial barrier and effectively reversed the gut microbiota dysbiosis, as evidenced by improvements in gut microbial gene richness, microbiota composition, and functional characteristics. The abundance of butyrate-producing strains, such as Bacteroides faecis, Butyomonas faecalis, Parabacteroides distasonis and Phocaeicola coprophilus is significantly increased, concurrently with the activation of the PPAR-γ signaling pathway. The findings of our study suggest that stachyose exhibits potential as a prebiotic agent for the prevention of gut microbiota dysbiosis and the intestinal epithelial barrier disruption in obese. The results of our study suggest that stachyose demonstrates potential as a prebiotic agent for mitigating gut microbiota dysbiosis and preserving the integrity of the intestinal epithelial barrier in individuals with MetS.

Experiment 1

Edit History Delete

Flag for review

Your review change was submittedDuplicate this Experiment Add a new Signature

Reviewed Marked as Reviewed by Svetlana up on 2024-6-9

Curated date: 2024/05/02

Curator: Joan Chuks

Revision editor(s): Joan Chuks, Scholastica

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: High fat diet HF - High fat diet,High fat diet (finding),High fat diet,high fat diet

Group 0 name Corresponds to the control (unexposed) group for case-control studies: High-Fat Diet fed Mice (HFD)

Group 1 name Corresponds to the case (exposed) group for case-control studies: Stachyose-treated High-Fat Diet fed Mice (STA)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Male C57BL/6J mice (18–20g, 6 weeks old) which were fed a High-Fat Diet (D12492-HFD diet, #XTHF60-1) with 60% of energy from fat, for 12 weeks and treatment with stachyose in the last 8 weeks (200 mg/ kg/day).

Group 0 sample size Number of subjects in the control (unexposed) group: 7

Group 1 sample size Number of subjects in the case (exposed) group: 7

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): None

Lab analysis

Sequencing type: WMS

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: DNBSEQ-T7

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Signature 1

Edit History Delete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by Svetlana up on 2024-6-9

Curated date: 2024/05/02

Curator: Joan Chuks

Revision editor(s): Joan Chuks

Source: Figure 5A

Description: Bacterial taxa identified by Linear discriminant analysis effect size (LEfSe) as differentially abundant in mice fed with Stachyose-treated High-Fat Diet compared to mice fed with High-Fat Diet.

Abundance in Group 1: increased abundance in Stachyose-treated High-Fat Diet fed Mice (STA)

NCBI	Quality Control	Links
Collinsella stercoris
Allobaculum mucilyticum
Thermophilibacter immobilis
Parolsenella catena
Parafannyhessea umbonata

Revision editor(s): Joan Chuks

Signature 2

Edit History Delete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by Svetlana up on 2024-6-9

Curated date: 2024/05/02

Curator: Joan Chuks

Revision editor(s): Joan Chuks

Source: Figure 5A

Description: Bacterial taxa identified by Linear discriminant analysis effect size (LEfSe) as differentially abundant in mice fed with Stachyose-treated High-Fat Diet compared to mice fed with High-Fat Diet.

Abundance in Group 1: decreased abundance in Stachyose-treated High-Fat Diet fed Mice (STA)

NCBI	Quality Control	Links
Escherichia marmotae

Revision editor(s): Joan Chuks