Weight gain in anorexia nervosa does not ameliorate the faecal microbiota, branched chain fatty acid profiles, and gastrointestinal complaints/Experiment 1
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< Weight gain in anorexia nervosa does not ameliorate the faecal microbiota, branched chain fatty acid profiles, and gastrointestinal complaints(Redirected from Weight gain in anorexia nervosa does not ameliorate the faecal microbiota, branched chain fatty acid profiles, and gastrointestinal complaints/Experiment 1)
Reviewed Marked as Reviewed by Lwaldron on 2022/04/6
Subjects
- Location of subjects
- Germany
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Anorexia nervosa anorexia nervosa,Anorexia nervosa
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- normal weight participants
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- anorexia patients before weight gain
- Group 0 sample size Number of subjects in the control (unexposed) group
- 55
- Group 1 sample size Number of subjects in the case (exposed) group
- 55
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 2 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.15
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Lwaldron on 2022/06/24
Source: figure 3
Description: gut microbiota of anorexia patients before and after weight gain compared to healthy controls
Abundance in Group 1: increased abundance in anorexia patients before weight gain
| NCBI | Quality Control | Links |
|---|---|---|
| Actinomycetota | ||
| Anaerostipes | ||
| Anaerotruncus | ||
| Bifidobacterium | ||
| Verrucomicrobiota | ||
| Clostridium |
Signature 2
Reviewed Marked as Reviewed by Lwaldron on 2022/06/24
Source: figure 3
Description: gut microbiota of anorexia patients before and after weight gain compared to healthy controls
Abundance in Group 1: decreased abundance in anorexia patients before weight gain
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidota | ||
| Gemmiger | ||
| Roseburia |
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