Gut Microbiome Alterations Precede Cerebral Amyloidosis and Microglial Pathology in a Mouse Model of Alzheimer's Disease
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Alzheimer's disease [X]Dementia in Alzheimer's disease,[X]Dementia in Alzheimer's disease (disorder),AD,AD - Alzheimer's disease,Alzheimer Dementia,Alzheimer dementia,Alzheimer Dementia, Presenile,ALZHEIMER DIS,Alzheimer Disease,Alzheimer disease,Alzheimer disease, familial,Alzheimer Type Dementia,Alzheimer's,Alzheimer's Dementia,Alzheimer's dementia,Alzheimer's disease,Alzheimer's disease (disorder),Alzheimer's disease, NOS,Alzheimers,Alzheimers Dementia,Alzheimers dementia,ALZHEIMERS DIS,Alzheimers disease,DAT - Dementia Alzheimer's type,Dementia in Alzheimer's disease,Dementia in Alzheimer's disease (disorder),Dementia in Alzheimer's disease, unspecified (disorder),Dementia of the Alzheimer's type,Dementia, Alzheimer Type,Dementia, Presenile,Dementia, Presenile Alzheimer,Disease, Alzheimer,Disease, Alzheimer's,Presenile Alzheimer Dementia,sporadic Alzheimer's disease,alzheimer's disease
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Wild-type (WT)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- APP/PS1
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Alzheimer's Disease (AD) mouse model 1-month-old
- Group 0 sample size Number of subjects in the control (unexposed) group
- 14
- Group 1 sample size Number of subjects in the case (exposed) group
- 21
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2.5
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Table S2
Description: Significantly different taxa between WT and AD (APP/PS1) mice
Abundance in Group 1: increased abundance in APP/PS1
NCBI | Quality Control | Links |
---|---|---|
Enterobacteriaceae |
Revision editor(s): AaishahM
Experiment 2
Subjects
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Alzheimer's Disease (AD) mouse model 2-month-old
- Group 0 sample size Number of subjects in the control (unexposed) group
- 17
- Group 1 sample size Number of subjects in the case (exposed) group
- 24
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Table S2
Description: Significantly different taxa between WT and AD (APP/PS1) mice
Abundance in Group 1: increased abundance in APP/PS1
NCBI | Quality Control | Links |
---|---|---|
Actinomycetota | ||
Akkermansia | ||
Allobaculum | ||
Alloprevotella | ||
Bifidobacterium | ||
Erysipelotrichaceae | ||
Prevotellaceae | ||
Verrucomicrobiaceae | ||
Verrucomicrobiota | ||
Thomasclavelia |
Revision editor(s): AaishahM
Experiment 3
Subjects
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Alzheimer's Disease (AD) mouse model 3-month-old
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Experiment 4
Subjects
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Alzheimer's Disease (AD) mouse model 6-month-old
- Group 0 sample size Number of subjects in the control (unexposed) group
- 31
- Group 1 sample size Number of subjects in the case (exposed) group
- 34
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Table S2
Description: Significantly different taxa between WT and AD (APP/PS1) mice
Abundance in Group 1: increased abundance in APP/PS1
Revision editor(s): AaishahM
Signature 2
Source: Table S2
Description: Significantly different taxa between WT and AD (APP/PS1) mice
Abundance in Group 1: decreased abundance in APP/PS1
NCBI | Quality Control | Links |
---|---|---|
Bacteroidaceae | ||
Bacteroides | ||
Escherichia/Shigella sp. | ||
Rikenellaceae | ||
g_Rikenellaceae_RC9_gut_groupg_Rikenellaceae_RC9_gut_group |
Revision editor(s): AaishahM
Experiment 5
Subjects
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Alzheimer's Disease (AD) mouse model 9-month-old
- Group 0 sample size Number of subjects in the control (unexposed) group
- 18
- Group 1 sample size Number of subjects in the case (exposed) group
- 18
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- increased
Signature 1
Source: Table S2
Description: Significantly different taxa between WT and AD (APP/PS1) mice
Abundance in Group 1: increased abundance in APP/PS1
Revision editor(s): AaishahM
Signature 2
Source: Table S2
Description: Significantly different taxa between WT and AD(APP/PS1) mice
Abundance in Group 1: decreased abundance in APP/PS1
NCBI | Quality Control | Links |
---|---|---|
Alistipes | ||
Atopostipes | ||
Carnobacteriaceae | ||
g_Lachnospiraceae_UCG-006g_Lachnospiraceae_UCG-006 | ||
g_[Eubacterium]_nodatum_groupg_[Eubacterium]_nodatum_group |
Revision editor(s): AaishahM
Experiment 6
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- A combination of groups WT 2m, 3m, 6m, 9m
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- WT 1m
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Wild-type mouse 1-month-old
- Group 0 sample size Number of subjects in the control (unexposed) group
- 83
- Group 1 sample size Number of subjects in the case (exposed) group
- 14
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Figure S3 A
Description: Differentially represented bacterial taxa of WT
Abundance in Group 1: increased abundance in WT 1m
Revision editor(s): AaishahM
Experiment 7
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- A combination of groups WT 1m, 3m, 6m, 9m
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- WT 2m
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Wild-type mouse 2-month-old
- Group 0 sample size Number of subjects in the control (unexposed) group
- 80
- Group 1 sample size Number of subjects in the case (exposed) group
- 17
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Figure S3 A
Description: Differentially represented bacterial taxa of WT
Abundance in Group 1: increased abundance in WT 2m
NCBI | Quality Control | Links |
---|---|---|
Muribaculaceae | ||
uncultured Muribaculaceae bacterium |
Revision editor(s): AaishahM
Experiment 8
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- A combination of groups WT 1m, 2m, 6m, 9m
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- WT 3m
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Wild-type mouse 3-month-old
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Figure S3 A
Description: Differentially represented bacterial taxa of WT
Abundance in Group 1: increased abundance in WT 3m
Revision editor(s): AaishahM
Experiment 9
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- A combination of groups WT 1m, 2m, 3m, 9m
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- WT 6m
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Wild-type mouse 6-month-old
- Group 0 sample size Number of subjects in the control (unexposed) group
- 66
- Group 1 sample size Number of subjects in the case (exposed) group
- 31
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Figure S3 A
Description: Differentially represented bacterial taxa of WT
Abundance in Group 1: increased abundance in WT 6m
NCBI | Quality Control | Links |
---|---|---|
Alloprevotella | ||
Allobaculum | ||
uncultured Peptococcaceae bacterium | ||
Peptococcaceae |
Revision editor(s): AaishahM
Experiment 10
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- A combination of groups WT 1m, 2m, 3m, 6m
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- WT 9m
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Wild-type mouse 9-month-old
- Group 0 sample size Number of subjects in the control (unexposed) group
- 79
- Group 1 sample size Number of subjects in the case (exposed) group
- 18
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- increased
Signature 1
Source: Figure S3 A
Description: Differentially represented bacterial taxa of WT
Abundance in Group 1: increased abundance in WT 9m
Revision editor(s): AaishahM
Experiment 11
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- A combination of groups APP/PS1 2m, 3m, 6m, 9m
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- APP/PS1 1m
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Alzheimer's Disease (AD) mouse model 1-month-old
- Group 0 sample size Number of subjects in the control (unexposed) group
- 100
- Group 1 sample size Number of subjects in the case (exposed) group
- 21
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Figure S3 B
Description: Differentially represented bacterial taxa of APP/PS1 1m
Abundance in Group 1: increased abundance in APP/PS1 1m
Revision editor(s): AaishahM
Experiment 12
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- A combination of groups APP/PS1 1m, 3m, 6m, 9m
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- APP/PS1 2m
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Alzheimer's Disease (AD) mouse model 2-month-old
- Group 0 sample size Number of subjects in the control (unexposed) group
- 97
- Group 1 sample size Number of subjects in the case (exposed) group
- 24
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Figure S3 B
Description: Differentially represented bacterial taxa of APP/PS1 2m
Abundance in Group 1: increased abundance in APP/PS1 2m
NCBI | Quality Control | Links |
---|---|---|
Staphylococcus | ||
Staphylococcaceae | ||
Bacillales | ||
Muribaculaceae | ||
uncultured Muribaculaceae bacterium |
Revision editor(s): AaishahM
Experiment 13
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- A combination of groups APP/PS1 1m, 2m, 6m, 9m
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- APP/PS1 3m
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Alzheimer's Disease (AD) mouse model 3-month-old
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Figure S3 B
Description: Differentially represented bacterial taxa of APP/PS1 3m
Abundance in Group 1: increased abundance in APP/PS1 3m
Revision editor(s): AaishahM
Experiment 14
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- A combination of groups APP/PS1 1m, 2m, 3m, 9m
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- APP/PS1 6m
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Alzheimer's Disease (AD) mouse model 6-month-old
- Group 0 sample size Number of subjects in the control (unexposed) group
- 87
- Group 1 sample size Number of subjects in the case (exposed) group
- 34
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Figure S3 B
Description: Differentially represented bacterial taxa of APP/PS1 6m
Abundance in Group 1: increased abundance in APP/PS1 6m
NCBI | Quality Control | Links |
---|---|---|
Allobaculum |
Revision editor(s): AaishahM
Experiment 15
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- A combination of groups APP/PS1 1m, 2m, 3m, 6m
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- APP/PS1 9m
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Alzheimer's Disease (AD) mouse model 9-month-old
- Group 0 sample size Number of subjects in the control (unexposed) group
- 103
- Group 1 sample size Number of subjects in the case (exposed) group
- 18
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- increased
Signature 1
Source: Figure S3 B
Description: Differentially represented bacterial taxa of APP/PS1 9m
Abundance in Group 1: increased abundance in APP/PS1 9m
Revision editor(s): AaishahM