Gut microbiota profiles in treatment-naïve children with attention deficit hyperactivity disorder/Experiment 1
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Attention deficit hyperactivity disorder ADD,ADDH,ADHD,ATTENTION DEFICIT DIS,ATTENTION DEFICIT DIS WITH HYPERACTIVITY,Attention Deficit Disorder,attention deficit disorder,Attention Deficit Disorder with Hyperactivity,Attention Deficit Disorders,Attention Deficit Disorders with Hyperactivity,ATTENTION DEFICIT HYPERACTIVITY DIS,attention deficit hyperactivity disorder, inattentive type,attention deficit hyperactivity disorder, inattentive/distractible type,attention deficit hyperactivity disorder, predominantly inattentive type,Attention Deficit Hyperactivity Disorders,Brain Dysfunction, Minimal,Deficit Disorder, Attention,Deficit Disorders, Attention,Disorder, Attention Deficit,Disorders, Attention Deficit,Dysfunction, Minimal Brain,hyperkinetic disorder,Hyperkinetic Syndrome,Minimal Brain Dysfunction,Syndromes, Hyperkinetic,Attention deficit hyperactivity disorder,attention deficit hyperactivity disorder
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy control
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- ADHD
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Treatment-Naive Children diagnosed with ADHD
- Group 0 sample size Number of subjects in the control (unexposed) group
- 32
- Group 1 sample size Number of subjects in the case (exposed) group
- 51
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 2 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Figure 1d , Figure S8
Description: (d) LEfSe identified the most differentially abundant taxons between HC and ADHD groups at genus level. Taxonomic cladogram obtained from LEfSe analysis of 16S sequences (relative abundance > 0.5%). HC-enriched taxa are indicated with a positive LDA score (green), and taxa enriched in ADHD have a negative score (red). Only taxa meeting an LDA significant threshold > 2 are shown
LEfSe identified the most differentially abundant taxons between HC and ADHD groups at family level. Taxonomic cladogram obtained from LEfSe analysis of 16S sequences (relative abundance >0.5%). (Red) ADHD taxa; (Green) taxa enriched in HCs. HC-enriched taxa are indicated with a positive LDA score (green), and taxa enriched in ADHD have a negative score (red). Only taxa meeting an LDA significant threshold >2 are shown.
Abundance in Group 1: increased abundance in ADHD
Revision editor(s): WikiWorks, Peace Sandy
Signature 2
Source: Figure1, Figure S8
Description: d) LEfSe identified the most differentially abundant taxons between HC and ADHD groups at genus level. Taxonomic cladogram obtained from LEfSe analysis of 16S sequences (relative abundance > 0.5%). HC-enriched taxa are indicated with a positive LDA score (green), and taxa enriched in ADHD have a negative score (red). Only taxa meeting an LDA significant threshold > 2 are shown
LEfSe identified the most differentially abundant taxons between HC and ADHD groups at family level. Taxonomic cladogram obtained from LEfSe analysis of 16S sequences (relative abundance >0.5%). (Red) ADHD taxa; (Green) taxa enriched in HCs. HC-enriched taxa are indicated with a positive LDA score (green), and taxa enriched in ADHD have a negative score (red). Only taxa meeting an LDA significant threshold >2 are shown.
Abundance in Group 1: decreased abundance in ADHD
| NCBI | Quality Control | Links |
|---|---|---|
| Alcaligenaceae | ||
| Dialister | ||
| Lachnoclostridium | ||
| Faecalibacterium | ||
| Lachnospira eligens | ||
| Sutterella | ||
| Acidaminococcus | ||
| Ruminiclostridium | ||
| Tyzzerella |
Revision editor(s): WikiWorks, Peace Sandy
