Comparative Studies of the Gut Microbiota in the Offspring of Mothers With and Without Gestational Diabetes/Experiment 8
From BugSigDB
Reviewed Marked as Reviewed by Folakunmi on 2024-11-25
Subjects
- Location of subjects
- Denmark
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Gestational diabetes diabetes in pregnancy,GDM,gestational diabetes,gestational diabetes mellitus,maternal gestational diabetes mellitus,Gestational diabetes
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normoglycaemic group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Gestational diabetes mellitus (GDM) group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Infants (9 months of age) born to mothers with gestational diabetes mellitus (GDM).
- Group 0 sample size Number of subjects in the control (unexposed) group
- 64
- Group 1 sample size Number of subjects in the case (exposed) group
- 32
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 2 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V1-V2
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- antibiotic therapy
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- unchanged
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-11-25
Source: Table S5
Description: OTUs differentially abundant in Infants (9 months of life) born to mothers with gestational diabetes mellitus (GDM) adjusted for perinatal antibiotic treatment (treated vs. untreated).
Abundance in Group 1: increased abundance in Gestational diabetes mellitus (GDM) group
| NCBI | Quality Control | Links |
|---|---|---|
| Alistipes | ||
| Blautia | ||
| Faecalibacterium |
Revision editor(s): Aleru Divine, AishatBolarinwa
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-11-25
Source: Table S5
Description: OTUs differentially abundant in Infants (9 months of life) born to mothers with gestational diabetes mellitus (GDM) adjusted for perinatal antibiotic treatment (treated vs. untreated).
Abundance in Group 1: decreased abundance in Gestational diabetes mellitus (GDM) group
| NCBI | Quality Control | Links |
|---|---|---|
| Escherichia/Shigella sp. | ||
| Prevotella | ||
| Rothia | ||
| Streptococcus | ||
| Veillonella |
Revision editor(s): Aleru Divine, AishatBolarinwa
