The Potential of Gut Microbiota in Prediction of Stroke-Associated Pneumonia

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Reviewed Marked as Reviewed by Svetlana up on 2024-10-22
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Li Z, Gu M, Sun H, Chen X, Zhou J, Zhang Y
Journal
Brain sciences
Year
2023
Keywords:
gut microbiota, predictor, short-chain fatty acids, stroke, stroke-associated pneumonia
BACKGROUND: Stroke-associated pneumonia (SAP) is a common stroke complication, and the changes in the gut microbiota composition may play a role. Our study aimed to evaluate the predictive ability of gut microbiota for SAP. METHODS: Acute ischemic stroke patients were prospectively enrolled and divided into two groups based on the presence or absence of SAP. The composition of gut microbiota was characterized by the 16S RNA Miseq sequencing. The gut microbiota that differed significantly between groups were incorporated into the conventional risk scores, the Acute Ischemic Stroke-Associated Pneumonia Score (AIS-APS), and the Age, Atrial fibrillation, Dysphagia, Sex, Stroke Severity Score (A2DS2). The predictive performances were assessed in terms of the area under the curve (AUC), the Net Reclassification Improvement (NRI), and the Integrated Discrimination Improvement (IDI) indices. RESULTS: A total of 135 patients were enrolled, of whom 43 had SAP (31%). The short-chain fatty acids (SCFAs)-producing bacteria, such as Bacteroides, Fusicatenibacter, and Butyricicoccus, were decreased in the SAP group. The integrated models showed better predictive ability for SAP (AUC = 0.813, NRI = 0.333, p = 0.052, IDI = 0.038, p = 0.018, for AIS-APS; AUC = 0.816, NRI = 0.575, p < 0.001, IDI = 0.043, p = 0.007, for A2DS2) in comparison to the differential genera (AUC = 0.699) and each predictive score (AUCAISAPS = 0.777; AUCA2DS2 = 0.777). CONCLUSIONS: The lower abundance of SCFAs-producing gut microbiota after acute ischemic stroke was associated with SAP and may play a role in SAP prediction.

Experiment 1


Reviewed Marked as Reviewed by Svetlana up on 2024-10-22

Curated date: 2024/10/03

Curator: Tosin

Revision editor(s): Tosin

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Ischemic stroke Ischaemic stroke,Ischemic stroke,ischemic stroke
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Patients without stroke-associated pneumonia (non-SAP)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Patients with stroke-associated pneumonia (SAP)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients who developed stroke-associated pneumonia after an acute ischemic stroke often due to immune suppression and difficulty swallowing.
Group 0 sample size Number of subjects in the control (unexposed) group
92
Group 1 sample size Number of subjects in the case (exposed) group
43
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
30 days

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-10-22

Curated date: 2024/10/04

Curator: Tosin

Revision editor(s): Tosin

Source: Figure 3b

Description: LEfSe of gut microbiota with significantly differential bacteria between two groups.(SAP and non-SAP). The analysis highlights the increased relative abundances of certain taxa in the SAP group. These taxa are identified as key microbial features distinguishing the SAP group from the NSAP group, potentially playing a role in the development of stroke-associated pneumonia.

Abundance in Group 1: increased abundance in Patients with stroke-associated pneumonia (SAP)

NCBI Quality ControlLinks
Bacilli
Butyrivibrio
Clostridium perfringens
Corynebacteriaceae
Corynebacterium
Lactobacillales
[Clostridium] innocuum
uncultured Lachnospiraceae bacterium
uncultured bacterium
uncultured organism

Revision editor(s): Tosin

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-10-22

Curated date: 2024/10/04

Curator: Tosin

Revision editor(s): Tosin

Source: Figure 3b

Description: LEfSe of gut microbiota with significantly differential between two groups.(SAP and Non SAP). The analysis highlights the decreased relative abundances of certain taxa in the SAP group, where they may indicate a protective role against the development of stroke-associated pneumonia.

Abundance in Group 1: decreased abundance in Patients with stroke-associated pneumonia (SAP)

NCBI Quality ControlLinks
Bacteroidales
Bacteroidota
Butyricicoccus
Fusicatenibacter
Negativicutes
Selenomonadales
Sutterellaceae
butyrate-producing bacterium L2-12
uncultured organism
Clostridium_XIVbClostridium_XIVb

Revision editor(s): Tosin

Experiment 2


Reviewed Marked as Reviewed by Svetlana up on 2024-10-22

Curated date: 2024/10/08

Curator: Tosin

Revision editor(s): Tosin

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Patients without stroke-associated Pnuemonia(non-SAP)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Patients with stroke-associated Pnuemonia(SAP)

Lab analysis

Statistical Analysis

Statistical test
Metastats
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
Not specified

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-10-22

Curated date: 2024/10/08

Curator: Tosin

Revision editor(s): Tosin

Source: Figure 2

Description: Gut microbiota with significantly different relative abundance between the two groups (SAP and non-SAP) at genus level in Metastats analysis where certain taxas were more enriched in the SAP group potentially contributing to the altered gut microbiota observed in the patients.

Abundance in Group 1: increased abundance in Patients with stroke-associated Pnuemonia(SAP)

NCBI Quality ControlLinks
Bacteroides
Butyricicoccus
Butyricimonas
Coprococcus
Fusicatenibacter
Clostridium_XIVbClostridium_XIVb

Revision editor(s): Tosin