The Potential of Gut Microbiota in Prediction of Stroke-Associated Pneumonia/Experiment 2
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-10-22
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Ischemic stroke Ischaemic stroke,Ischemic stroke,ischemic stroke
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Patients without stroke-associated Pnuemonia(non-SAP)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Patients with stroke-associated Pnuemonia(SAP)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients who developed stroke-associated pneumonia after an acute ischemic stroke often due to immune suppression and difficulty swallowing.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 92
- Group 1 sample size Number of subjects in the case (exposed) group
- 43
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 30 days
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Metastats
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-10-22
Source: Figure 2
Description: Gut microbiota with significantly different relative abundance between the two groups (SAP and non-SAP) at genus level in Metastats analysis where certain taxas were more enriched in the SAP group potentially contributing to the altered gut microbiota observed in the patients.
Abundance in Group 1: increased abundance in Patients with stroke-associated Pnuemonia(SAP)
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides | ||
| Butyricicoccus | ||
| Butyricimonas | ||
| Coprococcus | ||
| Fusicatenibacter | ||
| Clostridium_XIVbClostridium_XIVb |
Revision editor(s): Tosin
