Bacteroides uniformis degrades β-glucan to promote Lactobacillus johnsonii improving indole-3-lactic acid levels in alleviating colitis/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Colitis Colitides,colitis,colitis (disease),colon inflammation,inflammation of colon,Colitis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- DSS group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- BG + DSS group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Mice treated with β-glucan (BG) alongside dextran sulfate sodium (DSS) to study the potential therapeutic effects of BG on DSS-induced colitis.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Source: figure 2d
Description: Comparison of relative abundance of bacteria between DSS and BG + DSS group was calculated by linear discriminant analysis effect size (LEfSe). Taxa meeting an LDA score threshold > 2.0 is shown.
Abundance in Group 1: increased abundance in BG + DSS group
| NCBI | Quality Control | Links |
|---|---|---|
| Bacilli | ||
| Bacteroides uniformis | ||
| Lactobacillaceae | ||
| Lactobacillales | ||
| Lactobacillus | ||
| Porphyromonadaceae |
Revision editor(s): Appleeyes
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Source: figure 2d
Description: Comparison of relative abundance of bacteria between DSS and BG + DSS group was calculated by linear discriminant analysis effect size (LEfSe). Taxa meeting an LDA score threshold > 2.0 is shown.
Abundance in Group 1: decreased abundance in BG + DSS group
| NCBI | Quality Control | Links |
|---|---|---|
| Clostridia | ||
| Coriobacteriaceae | ||
| Coriobacteriales | ||
| Coriobacteriia | ||
| Lachnospiraceae | ||
| Actinomycetota |
Revision editor(s): Appleeyes
