Reduced microbiome alpha diversity in young patients with ADHD

Study information

Needs review

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

30001426

DOI Digital object identifier for electronic documents.

10.1371/journal.pone.0200728

URI

Authors

Prehn-Kristensen A, Zimmermann A, Tittmann L, Lieb W, Schreiber S, Baving L, Fischer A

Journal

PloS one

Year

2018

ADHD is a psychiatric disorder which is characterized by hyperactivity, impulsivity and attention problems. Due to recent findings of microbial involvement in other psychiatric disorders like autism and depression, a role of the gut microbiota in ADHD pathogenesis is assumed but has not yet been investigated. In this study, the gut microbiota of 14 male ADHD patients (mean age: 11.9 yrs.) and 17 male controls (mean age: 13.1 yrs.) was examined via next generation sequencing of 16S rDNA and analyzed for diversity and biomarkers. We found that the microbial diversity (alpha diversity) was significantly decreased in ADHD patients compared to controls (pShannon = 0.036) and that the composition (beta diversity) differed significantly between patients and controls (pANOSIM = 0.033, pADONIS = 0.006, pbetadisper = 0.002). In detail, the bacterial family Prevotellacae was associated with controls, while patients with ADHD showed elevated levels of Bacteroidaceae, and both Neisseriaceae and Neisseria spec. were found as possible biomarkers for juvenile ADHD. Our results point to a possible link of certain microbiota with ADHD, with Neisseria spec. being a very promising ADHD-associated candidate. This finding provides the basis for a systematic, longitudinal assessment of the role of the gut microbiome in ADHD, yielding promising potential for both prevention and therapeutic intervention.

Experiment 1

Edit History Delete

Mark reviewed

Your review change was submittedDuplicate this Experiment Add a new Signature

Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks

Subjects

Location of subjects: Germany

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Attention deficit hyperactivity disorder ADD,ADDH,ADHD,ATTENTION DEFICIT DIS,ATTENTION DEFICIT DIS WITH HYPERACTIVITY,Attention Deficit Disorder,attention deficit disorder,Attention Deficit Disorder with Hyperactivity,Attention Deficit Disorders,Attention Deficit Disorders with Hyperactivity,ATTENTION DEFICIT HYPERACTIVITY DIS,attention deficit hyperactivity disorder, inattentive type,attention deficit hyperactivity disorder, inattentive/distractible type,attention deficit hyperactivity disorder, predominantly inattentive type,Attention Deficit Hyperactivity Disorders,Brain Dysfunction, Minimal,Deficit Disorder, Attention,Deficit Disorders, Attention,Disorder, Attention Deficit,Disorders, Attention Deficit,Dysfunction, Minimal Brain,hyperkinetic disorder,Hyperkinetic Syndrome,Minimal Brain Dysfunction,Syndromes, Hyperkinetic,Attention deficit hyperactivity disorder,attention deficit hyperactivity disorder

Group 0 name Corresponds to the control (unexposed) group for case-control studies: controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: ADHD males

Group 0 sample size Number of subjects in the control (unexposed) group: 17

Group 1 sample size Number of subjects in the case (exposed) group: 14

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V1-V2

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Chao1 Abundance-based estimator of species richness: unchanged

Signature 1

Edit History Delete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: figure 3

Description: Differential abundance of taxa in male ADHD and healthy controls

Abundance in Group 1: increased abundance in ADHD males

NCBI	Quality Control	Links
Neisseriaceae
Neisseria
Bacteroides

Revision editor(s): WikiWorks

Signature 2

Edit History Delete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: figure 3

Description: Differential abundance of taxa in male ADHD and healthy controls

Abundance in Group 1: decreased abundance in ADHD males

NCBI	Quality Control	Links
Prevotellaceae
Christensenellaceae
Porphyromonadaceae
Prevotella
Parabacteroides

Revision editor(s): WikiWorks