Preconception helminth infection alters offspring microbiota and immune subsets in a mouse model
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Nyangahu DD, Darby M, Havyarimana E, Brown BP, Horsnell W, Jaspan HB
Journal
Parasite immunology
Year
2020
Keywords:
Nippostrongylus brasiliensis, CD4 T lymphocytes, Cell, animal model, parasite, tools and techniques
Both maternal microbiota and helminth infection may alter offspring immunity but the relationship between these is underexplored. We hypothesized that maternal helminth exposure prior to pregnancy has lasting consequences on offspring intestinal microbiota and consequent immunity. Female BALB/c adult mice were infected with 500L3 Nippostrongylus brasiliensis (N brasiliensis). Infection was cleared by ivermectin treatment, and mice were mated 3 weeks post-infection (NbM). Control mice were not infected but were exposed to ivermectin (NvM). We analysed maternal gut microbiota during pregnancy, breastmilk microbiota and offspring faecal microbiota and immunity 2 weeks after delivery. During pregnancy, NbM (Mothers previously infected with Nippostrongylus brasiliensis) displayed significantly altered stool bacterial communities (R2 = .242; P = .001), with increased abundance of Enterococcaceae versus NvM (Naive mothers). Similarly, we observed a profound impact on breastmilk microbiota in NbM vs NvM. Moreover, NbM pups showed significantly altered gut microbial communities at 14 days of age versus those born to NvM with increased relative abundance of Coriobacteriaceae and Micrococcaceae. These changes were associated with alterations in pup immunity including increased frequencies and numbers of activated CD4 T cells (CD4 + CD44hi) in NbM offspring spleens. Taken together, we show that preconception helminth infections impact offspring immunity possibly through alteration of maternal and offspring microbiota.
Experiment 1
Needs review
Subjects
- Location of subjects
- South Africa
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Intestinal helminthiasis intestine parasitic helminthiasis infectious disease,Intestinal helminthiasis,intestinal helminthiasis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Pregnant NvM
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Pregnant NbM
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- NbM comprises of female BALB/c adult mice who were infected with 500L3 Nippostrongylus brasiliensis (N brasiliensis).
- Group 0 sample size Number of subjects in the control (unexposed) group
- 4
- Group 1 sample size Number of subjects in the case (exposed) group
- 4
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V6
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- log transformation
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
Signature 1
Needs review
Source: Figure 1D, S1
Description: Differential abundant bacteria at different taxonomy level
Abundance in Group 1: increased abundance in Pregnant NbM
| NCBI | Quality Control | Links |
|---|---|---|
| Aerococcaceae | ||
| Alcaligenaceae | ||
| Bacillales | ||
| Burkholderiales | ||
| Enterococcaceae | ||
| Sphingobacteriaceae | ||
| Sphingobacteriales | ||
| Staphylococcaceae |
Revision editor(s): KateRasheed
Signature 2
Needs review
Source: Figure 1D, S1
Description: Differential abundant bacteria at different taxonomy level
Abundance in Group 1: decreased abundance in Pregnant NbM
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidaceae | ||
| Erysipelotrichaceae | ||
| Pasteurellaceae | ||
| Prevotellaceae |
Revision editor(s): KateRasheed
Experiment 2
Differences from previous experiment shown
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Stomach , Breast Anterior intestine,Gaster,Mesenteron,Stomach chamber,Ventriculus,Stomach,stomach,Mamma,Mammary part of chest,Mammary region,Breast,breast
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Helminthiasis, animal animal Helminthiases,animal helminthiasis,Helminthiases, animal,Helminthiasis, animal,helminthiasis, animal
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- NvM (Naive Mothers)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- NbM (Nippostrongylus brasiliensis Mothers)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- NbM - (Nippostrongylus brasiliensis Mothers) refers to the group of maternal mice that were previously infected with 500L3 larvae of Nippostrongylus brasiliensis. The infection was treated and cleared with ivermectin.
- Group 0 sample size Number of subjects in the control (unexposed) group
- "-4" can not be assigned to a declared number type with value 3.3-4
- Group 1 sample size Number of subjects in the case (exposed) group
- "-4" can not be assigned to a declared number type with value 3.3-4
Lab analysis
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Not specified
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- centered log-ratio
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Not specified
Experiment 3
Differences from previous experiment shown
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Breast , Milk Mamma,Mammary part of chest,Mammary region,Breast,breast,Mammary gland milk,Milk,milk
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- NbM - (Nippostrongylus brasiliensis Mothers) refers to the group of maternal mice that were previously infected with 500L3 larvae of Nippostrongylus brasiliensis. The infection was treated and cleared with ivermectin
Lab analysis
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
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