Preconception helminth infection alters offspring microbiota and immune subsets in a mouse model
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Nyangahu DD, Darby M, Havyarimana E, Brown BP, Horsnell W, Jaspan HB
Journal
Parasite immunology
Year
2020
Keywords:
Nippostrongylus brasiliensis, CD4 T lymphocytes, Cell, animal model, parasite, tools and techniques
Both maternal microbiota and helminth infection may alter offspring immunity but the relationship between these is underexplored. We hypothesized that maternal helminth exposure prior to pregnancy has lasting consequences on offspring intestinal microbiota and consequent immunity. Female BALB/c adult mice were infected with 500L3 Nippostrongylus brasiliensis (N brasiliensis). Infection was cleared by ivermectin treatment, and mice were mated 3 weeks post-infection (NbM). Control mice were not infected but were exposed to ivermectin (NvM). We analysed maternal gut microbiota during pregnancy, breastmilk microbiota and offspring faecal microbiota and immunity 2 weeks after delivery. During pregnancy, NbM (Mothers previously infected with Nippostrongylus brasiliensis) displayed significantly altered stool bacterial communities (R2 = .242; P = .001), with increased abundance of Enterococcaceae versus NvM (Naive mothers). Similarly, we observed a profound impact on breastmilk microbiota in NbM vs NvM. Moreover, NbM pups showed significantly altered gut microbial communities at 14 days of age versus those born to NvM with increased relative abundance of Coriobacteriaceae and Micrococcaceae. These changes were associated with alterations in pup immunity including increased frequencies and numbers of activated CD4 T cells (CD4 + CD44hi) in NbM offspring spleens. Taken together, we show that preconception helminth infections impact offspring immunity possibly through alteration of maternal and offspring microbiota.
Experiment 1
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Subjects
- Location of subjects
- South Africa
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Helminthiasis, animal animal Helminthiases,animal helminthiasis,Helminthiases, animal,Helminthiasis, animal,helminthiasis, animal
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Pregnant NvM
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Pregnant NbM
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Pregnant NbM are those previously infected with 500L3 Nippostrongylus brasiliensis (N brasiliensis) during pregnancy.
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V6
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- log transformation
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Source: Figure 1D, S1
Description: Differential abundant bacteria at different taxonomy level
Abundance in Group 1: increased abundance in Pregnant NbM
| NCBI | Quality Control | Links |
|---|---|---|
| Aerococcaceae | ||
| Alcaligenaceae | ||
| Bacillales | ||
| Burkholderiales | ||
| Enterococcaceae | ||
| Sphingobacteriaceae | ||
| Sphingobacteriales | ||
| Staphylococcaceae |
Revision editor(s): KateRasheed
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Source: Figure 1D, S1
Description: Differential abundant bacteria at different taxonomy level
Abundance in Group 1: decreased abundance in Pregnant NbM
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidaceae | ||
| Erysipelotrichaceae | ||
| Pasteurellaceae | ||
| Prevotellaceae | ||
| Pasteurellales | ||
| Erysipelotrichales |
Revision editor(s): KateRasheed
Experiment 2
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Differences from previous experiment shown
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Milk Mammary gland milk,Milk,milk
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- NvM breastmilk (Naive Mothers)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- NbM breastmilk (Nippostrongylus brasiliensis Mothers)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- NbM breastmilk refers to the breastmilk pellets from the stomachs of 14-day-old pups born to NbM. NbM - (Nippostrongylus brasiliensis Mothers) refers to the group of maternal mice that were previously infected with 500L3 larvae of Nippostrongylus brasiliensis. The infection was treated and cleared with ivermectin.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 5
- Group 1 sample size Number of subjects in the case (exposed) group
- 5
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Source: Figure 2D
Description: The breastmilk microbiota signatures were derived from breastmilk samples collected from the stomachs of 14-day-old pups born to mothers with and without preconception Nippostrongylus brasiliensis infections.
Abundance in Group 1: increased abundance in NbM breastmilk (Nippostrongylus brasiliensis Mothers)
| NCBI | Quality Control | Links |
|---|---|---|
| Enterobacteriaceae | ||
| Pseudomonadaceae |
Revision editor(s): Prolific
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Source: Figure 2D
Description: The breastmilk microbiota signatures were derived from breastmilk samples collected from the stomachs of 14-day-old pups born to mothers with and without preconception Nippostrongylus brasiliensis infections
Abundance in Group 1: decreased abundance in NbM breastmilk (Nippostrongylus brasiliensis Mothers)
| NCBI | Quality Control | Links |
|---|---|---|
| Aerococcaceae | ||
| Bacillaceae | ||
| Corynebacteriaceae | ||
| Planococcaceae |
Revision editor(s): Prolific
Experiment 3
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Differences from previous experiment shown
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- NvM pups
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- NbM pups
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- NbM pups are the babies given birth to by the NbM mothers.
- Group 0 sample size Number of subjects in the control (unexposed) group
- Not specified
- Group 1 sample size Number of subjects in the case (exposed) group
- Not specified
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Source: Figure 3D
Description: Differential abundant bacteria at different taxonomy level
Abundance in Group 1: increased abundance in NbM pups
| NCBI | Quality Control | Links |
|---|---|---|
| Erysipelotrichaceae | ||
| Coriobacteriaceae | ||
| Micrococcaceae | ||
| Comamonadaceae |
Revision editor(s): KateRasheed
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Source: Figure 3D
Description: Differential abundant bacteria at different taxonomy level
Abundance in Group 1: decreased abundance in NbM pups
| NCBI | Quality Control | Links |
|---|---|---|
| Clostridiaceae | ||
| Enterobacteriaceae | ||
| Corynebacteriaceae | ||
| Carnobacteriaceae |
Revision editor(s): KateRasheed
Experiment 4
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Differences from previous experiment shown
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Spleen Lien,Spleen,spleen
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Low CD4+CD44hi Group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- High CD4+CD44hi Group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- High refers to the CD4 + CD44hi frequency around the mean of samples from NbM pups.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 7
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Source: Figure 5H
Description: Differential abundance of microbiota in the CD4 + CD44hi frequency around the mean of samples from NbM pups.
Abundance in Group 1: increased abundance in High CD4+CD44hi Group
| NCBI | Quality Control | Links |
|---|---|---|
| Coprobacter | ||
| Gordonibacter | ||
| Clostridium IVClostridium IV |
Revision editor(s): Prolific, KateRasheed
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Source: Figure 5H
Description: Differential abundance of microbiota in the CD4 + CD44hi frequency around the mean of samples from NbM pups.
Abundance in Group 1: decreased abundance in High CD4+CD44hi Group
| NCBI | Quality Control | Links |
|---|---|---|
| Aerococcus | ||
| Mobilitalea |
Revision editor(s): Prolific, KateRasheed
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