Preconception helminth infection alters offspring microbiota and immune subsets in a mouse model/Experiment 2
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Subjects
- Location of subjects
- South Africa
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Milk Mammary gland milk,Milk,milk
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Helminthiasis, animal animal Helminthiases,animal helminthiasis,Helminthiases, animal,Helminthiasis, animal,helminthiasis, animal
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- NvM breastmilk (Naive Mothers)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- NbM breastmilk (Nippostrongylus brasiliensis Mothers)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- NbM breastmilk refers to the breastmilk pellets from the stomachs of 14-day-old pups born to NbM. NbM - (Nippostrongylus brasiliensis Mothers) refers to the group of maternal mice that were previously infected with 500L3 larvae of Nippostrongylus brasiliensis. The infection was treated and cleared with ivermectin.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 5
- Group 1 sample size Number of subjects in the case (exposed) group
- 5
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V6
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- log transformation
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Source: Figure 2D
Description: The breastmilk microbiota signatures were derived from breastmilk samples collected from the stomachs of 14-day-old pups born to mothers with and without preconception Nippostrongylus brasiliensis infections.
Abundance in Group 1: increased abundance in NbM breastmilk (Nippostrongylus brasiliensis Mothers)
| NCBI | Quality Control | Links |
|---|---|---|
| Enterobacteriaceae | ||
| Pseudomonadaceae |
Revision editor(s): Prolific
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Source: Figure 2D
Description: The breastmilk microbiota signatures were derived from breastmilk samples collected from the stomachs of 14-day-old pups born to mothers with and without preconception Nippostrongylus brasiliensis infections
Abundance in Group 1: decreased abundance in NbM breastmilk (Nippostrongylus brasiliensis Mothers)
| NCBI | Quality Control | Links |
|---|---|---|
| Aerococcaceae | ||
| Bacillaceae | ||
| Corynebacteriaceae | ||
| Planococcaceae |
Revision editor(s): Prolific
