Possible association of Firmicutes in the gut microbiota of patients with major depressive disorder

Study information

Reviewed Marked as Reviewed by Atrayees on 2023-8-18

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

30584306

DOI Digital object identifier for electronic documents.

10.2147/NDT.S188340

URI

Authors

Huang Y, Shi X, Li Z, Shen Y, Shi X, Wang L, Li G, Yuan Y, Wang J, Zhang Y, Zhao L, Zhang M, Kang Y, Liang Y

Journal

Neuropsychiatric disease and treatment

Year

2018

Keywords:

Firmicutes, brain–gut axis, depression, gut microbiota, short-chain fatty acids

Background: Gut microbiota can affect human behavior and mood in many ways. Several studies have shown that patients with depression were also accompanied with gut microbiota disorder, in which Firmicutes are related to the protective function of intestinal barrier. In this study, we explore the changes and effects of Firmicutes in the patients with major depressive disorder (MDD). Method: We recruited 54 subjects, including 27 patients with MDD. Fecal samples were collected for identification by 16S rRNA sequencing and bioinformatics analysis. Results: The study shows that the alpha diversity indices of MDD patients are lower than those of the healthy controls. Firmicutes is the most significantly decreased phylum in the MDD samples. There are totally 13 taxonomic biomarkers with P-value <0.01 from Firmicutes. There are differences in 17 KEGG pathways between the two groups. Conclusion: This study found that there is a significant disorder of gut microbiota in the patients with depression, in which the Firmicutes decreased significantly. Defects of the Firmicutes may lead to the depression in short-chain fatty acids, which could account for the physiological basis of low-level inflammation of depression. Limitations: This is a cross-sectional study and the sample size is comparatively small. Though several diet-related factors were controlled in the study, there is no quantified assessment of it.

Experiment 1

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Reviewed Marked as Reviewed by Atrayees on 2023-8-18

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Unipolar depression Depression,Depression, Emotional,Depression, Endogenous,Depression, Involutional,Depression, Neurotic,Depression, Unipolar,Depressions,Depressions, Emotional,Depressions, Endogenous,Depressions, Neurotic,Depressions, Unipolar,DEPRESSIVE DIS,DEPRESSIVE DIS MAJOR,Depressive Disorder,Depressive Disorder, Major,Depressive Disorders,Depressive Disorders, Major,Depressive Neuroses,Depressive Neurosis,Depressive Symptom,Depressive Symptoms,Depressive Syndrome,Depressive Syndromes,Disorder, Depressive,Disorder, Major Depressive,Disorders, Depressive,Disorders, Major Depressive,Emotional Depression,Emotional Depressions,Endogenous Depression,Endogenous Depressions,Involutional Depression,Involutional Psychoses,Involutional Psychosis,MAJOR DEPRESSIVE DIS,major depressive disorder,Major Depressive Disorders,Neuroses, Depressive,Neurosis, Depressive,Neurotic Depression,Neurotic Depressions,Paraphrenia, Involutional,Psychoses, Involutional,Psychosis, Involutional,Symptom, Depressive,Symptoms, Depressive,Syndrome, Depressive,Syndromes, Depressive,Unipolar Depressions,Unipolar depression,unipolar depression

Group 0 name Corresponds to the control (unexposed) group for case-control studies: controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: MDD

Group 0 sample size Number of subjects in the control (unexposed) group: 27

Group 1 sample size Number of subjects in the case (exposed) group: 27

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 6 months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Chao1 Abundance-based estimator of species richness: decreased

Signature 1

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Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Fig 1, fig 3b, figure 4

Description: Differential abundance of firmicutes in patients with major depressive disorder

Abundance in Group 1: increased abundance in MDD

NCBI	Quality Control	Links
Oxalobacter
Parvimonas
Bulleidia
Pseudomonas
Peptostreptococcus
Gemella

Revision editor(s): WikiWorks

Signature 2

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Flag for review

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Reviewed Marked as Reviewed by Atrayees on 2023-8-18

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Fig 1, fig 3b, figure 4

Description: Differential abundance of firmicutes in patients with major depressive disorder

Abundance in Group 1: decreased abundance in MDD

NCBI	Quality Control	Links
Bacillota
Lachnospiraceae
Faecalibacterium
Oscillospiraceae
Coprococcus
Blautia
Clostridiaceae
Dorea

Revision editor(s): WikiWorks