Gut microbiome and serum metabolome alterations associated with lactose intolerance (LI): a case‒control study and paired-sample study based on the American Gut Project (AGP)/Experiment 3
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-11-27
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Rattus norvegicus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Lactose intolerance lactase persistence,lactose intolerance,lactose intolerance (disease),LM - lactose malabsorption,Lactose intolerance
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- FMT-Healthy Controls group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- FMT-Lactose Intolerance group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Rats receiving gut microbiota from LI patients .
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Spearman Correlation
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- decreased
- Richness Number of species
- decreased
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-11-27
Source: Figure 7D
Description: Heatmap based on the relative abundance of the genera between FMT-HC (FMT-Healthy control group) and FMT-LI (FMT-Lactose intolerance group).
Abundance in Group 1: increased abundance in FMT-Lactose Intolerance group
| NCBI | Quality Control | Links |
|---|---|---|
| Coprobacillus | ||
| Dielma | ||
| Escherichia/Shigella sp. | ||
| Sutterella |
Revision editor(s): Nathcynthia, Tosin
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-11-27
Source: Figure 7D
Description: Heatmap based on the relative abundance of the genera between FMT-HC (Healthy control group) and FMT-LI (Lactose intolerance group).
Abundance in Group 1: decreased abundance in FMT-Lactose Intolerance group
| NCBI | Quality Control | Links |
|---|---|---|
| Anaerobutyricum hallii | ||
| Faecalibacterium | ||
| Fusicatenibacter |
Revision editor(s): Nathcynthia, Tosin
