Urogenital schistosomiasis is associated with signatures of microbiome dysbiosis in Nigerian adolescents

Study information

Reviewed Marked as Reviewed by Svetlana up on 2025-1-2

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

30696838

DOI Digital object identifier for electronic documents.

10.1038/s41598-018-36709-1

URI

Authors

Ajibola O, Rowan AD, Ogedengbe CO, Mshelia MB, Cabral DJ, Eze AA, Obaro S, Belenky P

Journal

Scientific reports

Year

2019

Urogenital schistosomiasis is a neglected tropical disease caused by the parasite Schistosoma haematobium, which resides in the vasculature surrounding the urogenital system. Previous work has suggested that helminthic infections can affect the intestinal microbiome, and we hypothesized that S. haematobium infection could result in an alteration of immune system-microbiota homeostasis and impact the composition of the gut microbiota. To address this question, we compared the fecal microbiomes of infected and uninfected schoolchildren from the Argungu Local Government Area of Kebbi State, Nigeria, detecting significant differences in community composition between the two groups. Most remarkably, we observed a decreased abundance of Firmicutes and increased abundance of Proteobacteria - a shift in community structure which has been previously associated with dysbiosis. More specifically, we detected a number of changes in lower taxa reminiscent of inflammation-associated dysbiosis, including decreases in Clostridiales and increases in Moraxellaceae, Veillonellaceae, Pasteurellaceae, and Desulfovibrionaceae. Functional potential analysis also revealed an enrichment in orthologs of urease, which has been linked to dysbiosis and inflammation. Overall, our analysis indicates that S. haematobium infection is associated with perturbations in the gut microbiota and may point to microbiome disruption as an additional consequence of schistosome infection.

Experiment 1

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Reviewed Marked as Reviewed by Svetlana up on 2025-1-2

Curated date: 2024/10/16

Curator: KateRasheed

Revision editor(s): KateRasheed, WikiWorks

Subjects

Location of subjects: Nigeria

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Schistosomiasis Bilharzia,Bilharziasis,bilharziasis,Katayama Fever,schistosomiasis,snail fever,Schistosomiasis

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Schistosomiasis negative

Group 1 name Corresponds to the case (exposed) group for case-control studies: Schistosomiasis positive

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Schistosomiasis positive are adolescents within the age of 11-15years that have been infected by Schistosoma.

Group 0 sample size Number of subjects in the control (unexposed) group: 25

Group 1 sample size Number of subjects in the case (exposed) group: 24

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: raw counts

Statistical test: DESeq2

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Richness Number of species: unchanged

Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample: unchanged

Signature 1

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Curated date: 2024/10/16

Curator: KateRasheed

Revision editor(s): KateRasheed, WikiWorks

Source: Figure 3(Figs. 3)

Description: Significant abundance of taxa in the infected adolescents.

Abundance in Group 1: increased abundance in Schistosomiasis positive

NCBI	Quality Control	Links
Acinetobacter
Alloprevotella
Bifidobacteriaceae
Deltaproteobacteria
Desulfovibrio
Desulfovibrionaceae
Desulfovibrionales
Dialister
Fusobacteriales
Fusobacteriia
Haemophilus
Megasphaera
Moraxellaceae
Negativicutes
Olsenella
Pasteurellaceae
Pasteurellales
Peptococcaceae
Peptococcus
Prevotella
Prevotellaceae
Pseudomonadales
Actinomycetes
Pseudomonadota
Selenomonadales
Veillonellaceae
uncultured Coriobacteriaceae bacterium
Acidimicrobiia

Revision editor(s): KateRasheed, WikiWorks

Signature 2

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Curated date: 2024/10/16

Curator: KateRasheed

Revision editor(s): KateRasheed, WikiWorks

Source: Figure 3(Figs. 3)

Description: Significant abundance of taxa in the infected adolescents.

Abundance in Group 1: decreased abundance in Schistosomiasis positive

NCBI	Quality Control	Links
Bacillota
Betaproteobacteria
Candidatus Gastranaerophilales
Candidatus Melainabacteria
Clostridia
Clostridium
Cyanobacteriota
Eubacteriales
Mollicutes
Mycoplasmatota
Oscillospiraceae incertae sedis
Parabacteroides
Peptostreptococcaceae
Peptostreptococcaceae incertae sedis
Porphyromonadaceae
Subdoligranulum
uncultured Erysipelotrichaceae bacterium
uncultured Mollicutes bacterium
uncultured Mycoplasmatota bacterium
uncultured rumen bacterium
uncultured bacterium

Revision editor(s): KateRasheed, WikiWorks