Faecal Microbiota Characterisation of Potamochoerus porcus Living in a Controlled Environment/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2024-10-25

Curated date: 2024/10/17

Curator: Prolific

Revision editor(s): Prolific, Aleru Divine

Subjects

Location of subjects
Italy
Host species Species from which microbiome was sampled. Contact us to have more species added.
Potamochoerus porcus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Environmental factor environment factor,Environmental factor,environmental factor
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Potamochoerus porcus housed in Verona
Group 1 name Corresponds to the case (exposed) group for case-control studies
Potamochoerus porcus housed in Rome
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Potamochoerus porcus housed in Bioparco, Rome, Italy. This group included 2 adult individuals.
Group 0 sample size Number of subjects in the control (unexposed) group
3
Group 1 sample size Number of subjects in the case (exposed) group
2
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
2 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
DESeq2
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
unchanged
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
decreased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-10-25

Curated date: 2024/10/17

Curator: Prolific

Revision editor(s): Prolific, Aleru Divine

Source: Table 3 and Table S2

Description: Key microbial taxa that were differentially abundant in gut microbiota composition of Red River Hogs (Potamochoerus porcus) housed in Rome and Verona parks.

Abundance in Group 1: increased abundance in Potamochoerus porcus housed in Rome

NCBI Quality ControlLinks
Alloprevotella
Bacteroidota
Cellulosilyticum
Elusimicrobiota
Fibrobacterota
Lachnospira
Paludibacteraceae
Pseudomonadota
Sutterella
Sutterellaceae
Thermodesulfobacteriota
Thermoplasmatota
uncultured Eubacteriales bacterium
uncultured Lachnospiraceae bacterium
uncultured Prevotellaceae bacterium
Candidatus Paracaedibacteraceae

Revision editor(s): Prolific, Aleru Divine

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-10-25

Curated date: 2024/10/21

Curator: Prolific

Revision editor(s): Prolific, Aleru Divine

Source: Table 3 and Table S2

Description: Key microbial taxa that were differentially abundant in gut microbiota composition of Red River Hogs (Potamochoerus porcus) housed in Rome and Verona parks.

Abundance in Group 1: decreased abundance in Potamochoerus porcus housed in Rome

NCBI Quality ControlLinks
Spirochaetaceae
Akkermansia
Defluviitaleaceae
unclassified Bacteroidales
Lactobacillaceae
Corynebacteriaceae
Treponema
Blautia
Candidatus Soleaferrea
unclassified Erysipelotrichaceae
Anaerostipes
Dorea
Papillibacter
unclassified Lachnospiraceae
Lactobacillus
unclassified Rikenellaceae
Corynebacterium
Bacillota
Spirochaetota
Verrucomicrobiota
Actinomycetota
Synergistota
Methanobacteriota
Planctomycetota
Patescibacteria group

Revision editor(s): Prolific, Aleru Divine