Antenatal gut microbiome profiles and effect on pregnancy outcome in HIV infected and HIV uninfected women in a resource limited setting/Experiment 3
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-12-11
Subjects
- Location of subjects
- Zimbabwe
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Viral load viral burden,viral titer,viral titre,Viral load,viral load
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Human immunodeficiency virus (HIV) virally suppressed pregnant women (Viral Load ≤ 1000 copies/ml)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Human immunodeficiency virus (HIV) virally un-suppressed pregnant women (Viral Load > 1000 copies/ml)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- HIV Un-suppressed pregnant women with viral load greater than 1000 cps/ml.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 29
- Group 1 sample size Number of subjects in the case (exposed) group
- 5
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V5-V6
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Ion Torrent
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- arcsine square-root
- Statistical test
- MaAsLin2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-12-11
Source: Figure 3b
Description: Taxonomic differences between the gut microbiota of groups stratified by viral load.
Abundance in Group 1: increased abundance in Human immunodeficiency virus (HIV) virally un-suppressed pregnant women (Viral Load > 1000 copies/ml)
| NCBI | Quality Control | Links |
|---|---|---|
| Prevotella | ||
| unclassified Paraprevotella |
Revision editor(s): Tosin
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-12-11
Source: Figure 3b
Description: Taxonomic differences between the gut microbiota of groups stratified by viral load.
Abundance in Group 1: decreased abundance in Human immunodeficiency virus (HIV) virally un-suppressed pregnant women (Viral Load > 1000 copies/ml)
| NCBI | Quality Control | Links |
|---|---|---|
| Clostridium |
Revision editor(s): Tosin
