Sampling from four geographically divergent young female populations demonstrates forensic geolocation potential in microbiomes/Experiment 3

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2024-11-18

Curated date: 2024/11/12

Curator: KateRasheed

Revision editor(s): KateRasheed

Subjects

Location of subjects
Barbados
Chile
South Africa
Thailand
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Lifestyle measurement Lifestyle measurement,lifestyle measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Barbados, Chile, South Africa
Group 1 name Corresponds to the case (exposed) group for case-control studies
Thailand
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Thailand refers to the samples from healthy females aged between 18 and 26 years, based in Thailand.
Group 0 sample size Number of subjects in the control (unexposed) group
138
Group 1 sample size Number of subjects in the case (exposed) group
68
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
Participants currently taking antibiotics were excluded from the study.

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
DESeq2
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes


Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-11-18

Curated date: 2024/11/12

Curator: KateRasheed

Revision editor(s): KateRasheed

Source: Table S8

Description: Differentially abundant taxa in stool samples for Thailand location.

Abundance in Group 1: increased abundance in Thailand

NCBI Quality ControlLinks
Escherichia coli
Fusobacterium mortiferum
Phocaeicola coprocola DSM 17136
Phocaeicola massiliensis
Phocaeicola plebeius
Ruminococcus sp. 5_1_39BFAA
unclassified Alistipes
unclassified Sutterella
uncultured meganomonasuncultured meganomonas
Parabacteroides merdae

Revision editor(s): KateRasheed

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-11-18

Curated date: 2024/11/12

Curator: KateRasheed

Revision editor(s): KateRasheed

Source: Table S8

Description: Differentially abundant taxa in stool samples for Thailand location.

Abundance in Group 1: decreased abundance in Thailand

NCBI Quality ControlLinks
uncultured Pseudobutyrivibrio sp.
uncultured Prevotellaceae bacterium
Ruminococcaceae_UCG-002Ruminococcaceae_UCG-002
unclassified Akkermansia
Bacteroides ovatus
Uncultured prevotellaUncultured prevotella
unclassified Ruminococcus
unclassified Subdoligranulum
Ruminococcaceae_UCG-014Ruminococcaceae_UCG-014
unclassified Blautia
Ruminococcus bicirculans (ex Liu et al. 2021)
uncultured Bacteroides sp.
unclassified Faecalibacterium
uncultured Christensenellaceae bacterium
uncultured Dorea sp.
Ruminococcaceae_UCG-005Ruminococcaceae_UCG-005

Revision editor(s): KateRasheed