Sampling from four geographically divergent young female populations demonstrates forensic geolocation potential in microbiomes/Experiment 9

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Reviewed Marked as Reviewed by Svetlana up on 2024-11-18

Curated date: 2024/11/12

Curator: KateRasheed

Revision editor(s): KateRasheed

Subjects

Location of subjects
Barbados
Chile
South Africa
Thailand
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Cheek Buccae,Jowl,Cheek,cheek
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Lifestyle measurement Lifestyle measurement,lifestyle measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Barbados, South Africa, Thailand
Group 1 name Corresponds to the case (exposed) group for case-control studies
Chile
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Chile refers to the samples from healthy females aged between 18 and 26 years, based in Chile.
Group 0 sample size Number of subjects in the control (unexposed) group
137
Group 1 sample size Number of subjects in the case (exposed) group
69
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
Participants currently taking antibiotics were excluded from the study.

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
DESeq2
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes


Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-11-18

Curated date: 2024/11/12

Curator: KateRasheed

Revision editor(s): KateRasheed

Source: Table S14

Description: Differentially abundant taxa in oral samples between Chile and other locations.

Abundance in Group 1: increased abundance in Chile

NCBI Quality ControlLinks
unclassified Streptococcus

Revision editor(s): KateRasheed

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-11-18

Curated date: 2024/11/12

Curator: KateRasheed

Revision editor(s): KateRasheed

Source: Table S14

Description: Differentially abundant taxa in oral samples between Chile and other locations.

Abundance in Group 1: decreased abundance in Chile

NCBI Quality ControlLinks
Neisseria oralis
Prevotella melaninogenica
Prevotella pallens
unclassified Actinomyces
unclassified Prevotella
unclassified Veillonella
uncultured Bacteroidota bacterium
Prevotella_7_unclassifiedPrevotella_7_unclassified
Streptococcus thermophilus

Revision editor(s): KateRasheed