Gut microbiomes of agropastoral children from the Adadle region of Ethiopia reflect their unique dietary habits/Experiment 1
From BugSigDB
Subjects
- Location of subjects
- Ethiopia
- Madagascar
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Population Population,population
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Madagascar
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Adadle
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- 2-5 year old children from the Adadle woreda district in Ethiopia, whose samples were sequenced using Primer 1 set (V4 501-508F/701-712R).
- Group 0 sample size Number of subjects in the control (unexposed) group
- 431
- Group 1 sample size Number of subjects in the case (exposed) group
- 54
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Source: Figure S6 B
Description: LEfSe analysis using primer set 1 data showing ASV bacterial families associated with Adadle or Madagascar.
Abundance in Group 1: increased abundance in Adadle
Revision editor(s): YokoC
Signature 2
Source: Figure S6 B
Description: LEfSe analysis using primer set 1 data showing ASV bacterial families associated with Adadle or Madagascar.
Abundance in Group 1: decreased abundance in Adadle
Revision editor(s): YokoC
