Gut microbiomes of agropastoral children from the Adadle region of Ethiopia reflect their unique dietary habits/Experiment 10

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2025-1-22

Curated date: 2024/10/25

Curator: YokoC

Revision editor(s): YokoC

Subjects

Location of subjects
Ethiopia
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
COPRO-seq COPRO-seq,cOPRO-seq
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Primer set 2
Group 1 name Corresponds to the case (exposed) group for case-control studies
mOTUs2
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
mOTUs2 taxonomic profiling of shotgun metagenomic samples from Adadle woreda 2-5 year old children.
Group 0 sample size Number of subjects in the control (unexposed) group
6
Group 1 sample size Number of subjects in the case (exposed) group
6

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
log transformation
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes


Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-1-22

Curated date: 2024/10/25

Curator: YokoC

Revision editor(s): YokoC

Source: Figure S8 B

Description: Boxplots of families with a significantly different abundance (Wilcoxon rank test) between primer set 2 and mOTUs2.

Abundance in Group 1: increased abundance in mOTUs2

NCBI Quality ControlLinks
Bacteria incertae sedis
Clostridiaceae
Corynebacteriaceae
Eubacteriaceae
Eubacteriales Family XIII. Incertae Sedis
Eubacteriales incertae sedis
Gemellaceae
Micrococcaceae
Propionibacteriaceae
Sutterellaceae
unclassified Bacilli
unclassified Bacillota
unclassified Bacteria
unclassified Candidatus Saccharibacteria
unclassified Eubacteriales
unclassified Lactobacillales

Revision editor(s): YokoC

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-1-22

Curated date: 2024/10/25

Curator: YokoC

Revision editor(s): YokoC

Source: Figure S8 B

Description: Boxplots of families with a significantly different abundance (Wilcoxon rank test) between primer set 2 and mOTUs2.

Abundance in Group 1: decreased abundance in mOTUs2

NCBI Quality ControlLinks
Anaerovoracaceae

Revision editor(s): YokoC