Gut microbiomes of agropastoral children from the Adadle region of Ethiopia reflect their unique dietary habits/Experiment 11

From BugSigDB


Needs review

Curated date: 2024/10/30

Curator: YokoC

Revision editor(s): YokoC

Subjects

Location of subjects
Central African Republic
Ethiopia
Madagascar
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Population Population,population
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Cluster P+
Group 1 name Corresponds to the case (exposed) group for case-control studies
Cluster P-
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
One of two clusters based on bacterial taxa abundance, determined by the use of Euclidean distances and Ward's linkage method on data from primer 1 set.
Group 0 sample size Number of subjects in the control (unexposed) group
526
Group 1 sample size Number of subjects in the case (exposed) group
233

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes


Signature 1

Needs review

Curated date: 2024/10/31

Curator: YokoC

Revision editor(s): YokoC

Source: Figure 3 C

Description: Heatmap of the most abundant species with significantly different relative abundance between two clusters (P+ and P-) in the primer 1 set.

Abundance in Group 1: increased abundance in Cluster P-

NCBI Quality ControlLinks
Bifidobacterium catenulatum
Blautia obeum
Collinsella aerofaciens
Roseburia inulinivorans
Streptococcus lutetiensis
Escherichia/Shigella coliEscherichia/Shigella coli
Erysipelotrichaceae UCG-003Erysipelotrichaceae UCG-003
Prevotella sp.
Prevotellaceae bacterium
Candidatus bacilloplasma bacteriumCandidatus bacilloplasma bacterium

Revision editor(s): YokoC

Signature 2

Needs review

Curated date: 2024/10/31

Curator: YokoC

Revision editor(s): YokoC

Source: Figure 3C

Description: Heatmap of the most abundant species with significantly different relative abundance between two clusters (P+ and P-) in the primer 1 set.

Abundance in Group 1: decreased abundance in Cluster P-

NCBI Quality ControlLinks
Leyella stercorea
Prevotella 9 copriPrevotella 9 copri
Alloprevotella sp.
Dialister sp.
Phascolarctobacterium sp.
Succinivibrio sp.
unclassified Prevotella 9unclassified Prevotella 9

Revision editor(s): YokoC