Gut microbiomes of agropastoral children from the Adadle region of Ethiopia reflect their unique dietary habits/Experiment 3

From BugSigDB


Needs review

Curated date: 2024/10/23

Curator: YokoC

Revision editor(s): YokoC

Subjects

Location of subjects
Ethiopia
Sweden
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Population Population,population
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Industrial Lifestyle
Group 1 name Corresponds to the case (exposed) group for case-control studies
Adadle Agropastoral Lifestyle
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
2-5 year old children from the Adadle woreda district in Ethiopia with an Agropastoral lifestyle, whose samples were sequenced using Primer 2 set (V4 515F/806R). Lifestyle classifications of the different populations were based on the publications used for the datasets.
Group 0 sample size Number of subjects in the control (unexposed) group
484
Group 1 sample size Number of subjects in the case (exposed) group
13

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2


Signature 1

Needs review

Curated date: 2024/10/23

Curator: YokoC

Revision editor(s): YokoC

Source: Figure S6 D

Description: LEfSe analysis showing bacterial families associated with agropastoral and industrial lifestyles.

Abundance in Group 1: increased abundance in Adadle Agropastoral Lifestyle

NCBI Quality ControlLinks
Anaerovoracaceae
Atopobiaceae
Bacillaceae
Coprobacillaceae
Coriobacteriaceae
Enterococcaceae
Erysipelotrichaceae
Monoglobaceae
Prevotellaceae
Streptococcaceae
Succinivibrionaceae

Revision editor(s): YokoC

Signature 2

Needs review

Curated date: 2024/10/23

Curator: YokoC

Revision editor(s): YokoC

Source: Figure S6 D

Description: LEfSe analysis showing bacterial families associated with agropastoral and industrial lifestyles.

Abundance in Group 1: decreased abundance in Adadle Agropastoral Lifestyle

NCBI Quality ControlLinks
Akkermansiaceae
Bacteroidaceae
Barnesiellaceae
Bifidobacteriaceae
Clostridiaceae
Defluviitaleaceae
Desulfovibrionaceae
Marinifilaceae
Oscillospiraceae
Pasteurellaceae
Peptostreptococcaceae
Porphyromonadaceae
Rikenellaceae
Sutterellaceae
Tannerellaceae
Veillonellaceae

Revision editor(s): YokoC