Gut microbiomes of agropastoral children from the Adadle region of Ethiopia reflect their unique dietary habits/Experiment 6

From BugSigDB


Needs review

Curated date: 2024/10/24

Curator: YokoC

Revision editor(s): YokoC

Subjects

Location of subjects
Ethiopia
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
COPRO-seq COPRO-seq,cOPRO-seq
Group 0 name Corresponds to the control (unexposed) group for case-control studies
mOTUs2
Group 1 name Corresponds to the case (exposed) group for case-control studies
MetaPhlAn3
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
MetaPhlan3 taxonomic profiling of shotgun metagenomic samples from Adadle woreda 2-5 year old children.
Group 0 sample size Number of subjects in the control (unexposed) group
15
Group 1 sample size Number of subjects in the case (exposed) group
15

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
log transformation
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes


Signature 1

Needs review

Curated date: 2024/10/24

Curator: YokoC

Revision editor(s): YokoC

Source: Figure S7 A

Description: Boxplots of species with a significantly different abundance (Wilcoxon rank test) between the two profiling tools, mOTUs2 and MetaPhlAn3.

Abundance in Group 1: increased abundance in MetaPhlAn3

NCBI Quality ControlLinks
Actinomyces oris
Actinomyces sp. HMSC035G02
Actinomyces sp. oral taxon 181
Agathobaculum butyriciproducens
Bacteroides uniformis
Collinsella intestinalis
Collinsella stercoris
Eggerthella lenta
Fusicatenibacter saccharivorans
Gemmiger formicilis
Parabacteroides distasonis
Roseburia sp. CAG:471
Ruthenibacterium lactatiformans
Slackia isoflavoniconvertens
[Collinsella] massiliensis

Revision editor(s): YokoC

Signature 2

Needs review

Curated date: 2024/10/24

Curator: YokoC

Revision editor(s): YokoC

Source: Figure S7 A

Description: Boxplots of species with a significantly different abundance (Wilcoxon rank test) between the two profiling tools, mOTUs2 and MetaPhlAn3..

Abundance in Group 1: decreased abundance in MetaPhlAn3

NCBI Quality ControlLinks
Actinomyces dentalis
Actinomyces sp. ICM39
Actinomyces viscosus
Arachnia propionica
Bacilli bacterium
Bacillota bacterium
Bifidobacterium catenulatum subsp. kashiwanohense
Blautia sp. KLE 1732
Candidatus Nanosynbacter lyticus
Eggerthellaceae bacterium
Lachnospiraceae bacterium
Lactobacillales bacterium
Oscillospiraceae bacterium
Peptostreptococcaceae bacterium
Senegalimassilia anaerobia
Streptococcus gallolyticus
Streptococcus intermedius
Streptococcus sp.
Streptococcus sp. HSISM1
bacterium LF-3
uncultured Butyricicoccus sp.
uncultured Candidatus Saccharibacteria bacterium
uncultured Clostridium sp.
uncultured Collinsella sp.
uncultured Eubacterium sp.
uncultured Faecalibacterium sp.
uncultured Mogibacterium sp.
uncultured Prevotella sp.
uncultured Streptococcus sp.
uncultured Sutterella sp.
Eggerthella lenta [C Clostridioides difficile]Eggerthella lenta [C Clostridioides difficile]<i class="fas text- fa-comment" title="The part "]" of the query was not understood.</br>Results might not be as expected. - Review">
Actinomyces sp. [ICM58/oral taxon 172]Actinomyces sp. [ICM58/oral taxon 172]<i class="fas text- fa-comment" title="The part "]" of the query was not understood.</br>Results might not be as expected. - Review">
Actinomyces sp. [oral taxon 181/ph3]Actinomyces sp. [oral taxon 181/ph3]<i class="fas text- fa-comment" title="The part "]" of the query was not understood.</br>Results might not be as expected. - Review">
Streptococcus ilei
Phocaeicola sp.
Bacteroides sp.

Revision editor(s): YokoC