Microbial gut evaluation in an angolan paediatric population with sickle cell disease/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-11-27
Subjects
- Location of subjects
- Angola
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Sickle cell anemia drepanocytosis,Hb SC disease,Hb-S/Hb-C disease,Hb-SS disease without crisis,HbS disease,Hemoglobin S disease,Hemoglobin S disease without crisis,hemoglobin SC disease,sickle cell anemia,Sickle cell disease,sickle cell disease,sickle-cell/Hb-C disease without crisis,sickling disorder due to Hemoglobin S,Sickle cell anemia
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy Siblings
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Sickle Cell Anaemia Children
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Angolan children between 3 and 14 years with Sickle Cell Anemia
- Group 0 sample size Number of subjects in the control (unexposed) group
- 36
- Group 1 sample size Number of subjects in the case (exposed) group
- 36
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Welch's T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-11-27
Source: Figure 4
Description: Differential abundance in the microbiota of sickle cell anaemia (SCA) children compared to their siblings(controls).
Abundance in Group 1: increased abundance in Sickle Cell Anaemia Children
Revision editor(s): Aleru Divine
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-11-27
Source: Figure 4
Description: Differential abundance in the microbiota of sickle cell anaemia (SCA) children compared to their siblings(controls).
Abundance in Group 1: decreased abundance in Sickle Cell Anaemia Children
Revision editor(s): Aleru Divine
