Visceral adiposity in postmenopausal women is associated with a pro-inflammatory gut microbiome and immunogenic metabolic endotoxemia

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Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Authors
Gaber M, Wilson AS, Millen AE, Hovey KM, LaMonte MJ, Wactawski-Wende J, Ochs-Balcom HM, Cook KL
Journal
Microbiome
Year
2024
Keywords:
Aging, Inflammation, Leaky gut, Lipopolysaccharide, Menopause, Metabolic endotoxemia, Microbiome, Obesity, Women’s Health Initiative
BACKGROUND: Obesity, and in particular abdominal obesity, is associated with an increased risk of developing a variety of chronic diseases. Obesity, aging, and menopause are each associated with differential shifts in the gut microbiome. Obesity causes chronic low-grade inflammation due to increased lipopolysaccharide (LPS) levels which is termed "metabolic endotoxemia." We examined the association of visceral adiposity tissue (VAT) area, circulating endotoxemia markers, and the gut bacterial microbiome in a cohort of aged postmenopausal women. METHODS: Fifty postmenopausal women (mean age 78.8 ± 5.3 years) who had existing adipose measurements via dual x-ray absorptiometry (DXA) were selected from the extremes of VAT: n = 25 with low VAT area (45.6 ± 12.5 cm2) and n = 25 with high VAT area (177.5 ± 31.3 cm2). Dietary intake used to estimate the Healthy Eating Index (HEI) score was assessed with a food frequency questionnaire. Plasma LPS, LPS-binding protein (LBP), anti-LPS antibodies, anti-flagellin antibodies, and anti-lipoteichoic acid (LTA) antibodies were measured by ELISA. Metagenomic sequencing was performed on fecal DNA. Female C57BL/6 mice consuming a high-fat or low-fat diet were treated with 0.4 mg/kg diet-derived fecal isolated LPS modeling metabolic endotoxemia, and metabolic outcomes were measured after 6 weeks. RESULTS: Women in the high VAT group showed increased Proteobacteria abundance and a lower Firmicutes/Bacteroidetes ratio. Plasma LBP concentration was positively associated with VAT area. Plasma anti-LPS, anti-LTA, and anti-flagellin IgA antibodies were significantly correlated with adiposity measurements. Women with high VAT showed significantly elevated LPS-expressing bacteria compared to low VAT women. Gut bacterial species that showed significant associations with both adiposity and inflammation (anti-LPS IgA and LBP) were Proteobacteria (Escherichia coli, Shigella spp., and Klebsiella spp.) and Veillonella atypica. Healthy eating index (HEI) scores negatively correlated with % body fat and anti-LPS IgA antibodies levels. Preclinical murine model showed that high-fat diet-fed mice administered a low-fat diet fecal-derived LPS displayed reduced body weight, decreased % body fat, and improved glucose tolerance test parameters when compared with saline-injected or high-fat diet fecal-derived LPS-treated groups consuming a high-fat diet. CONCLUSIONS: Increased VAT in postmenopausal women is associated with elevated gut Proteobacteria abundance and immunogenic metabolic endotoxemia markers. Low-fat diet-derived fecal-isolated LPS improved metabolic parameters in high-fat diet-fed mice giving mechanistic insights into potential pro-health signaling mediated by under-acylated LPS isoforms. Video Abstract.

Experiment 1


Needs review

Curated date: 2024/10/24

Curator: Vandana Maddi

Revision editor(s): Vandana Maddi

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Visceral adipose tissue measurement , Gut microbiome measurement Visceral adipose tissue measurement,visceral adipose tissue measurement,Gut microbiome measurement,gut microbiome measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Low VAT group (Control/Unexposed group).
Group 1 name Corresponds to the case (exposed) group for case-control studies
High VAT group (Case/Exposed group)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
The high VAT group represents women with elevated visceral adipose tissue, which is associated with pro-inflammatory gut microbiome characteristics and metabolic endotoxemia
Group 0 sample size Number of subjects in the control (unexposed) group
25
Group 1 sample size Number of subjects in the case (exposed) group
25
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
Participants with detectable antibiotics in plasma were excluded from the analysis to prevent confounding effects on gut microbiome composition. This included antibiotics like amoxicillin and clindamycin.

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V9
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
log transformation
Statistical test
LEfSe
Mann-Whitney (Wilcoxon)
PERMANOVA
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2.0
Matched on Factors on which subjects have been matched on in a case-control study
Matched on: "VAT (visceral adipose tissue) area" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.VAT (visceral adipose tissue) area
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
Confounders controlled for: "antibiotic use" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.antibiotic use, Confounders controlled for: "dietary intake" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.dietary intake, Confounders controlled for: "hormone therapy usage" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.hormone therapy usage

Alpha Diversity

Pielou Quantifies how equal the community is numerically
unchanged
Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
increased
Richness Number of species
unchanged
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
unchanged

Signature 1

Needs review

Curated date: 2024/10/24

Curator: Vandana Maddi

Revision editor(s): Vandana Maddi

Source: Fecal microbiome of postmenopausal women

Description: Increased abundance of Proteobacteria, a phylum of gram-negative bacteria linked to inflammation, in women with high visceral adiposity tissue (VAT).

Abundance in Group 1: increased abundance in High VAT group (Case/Exposed group)

NCBI Quality ControlLinks
Enterobacteriaceae
Escherichia coli
Pseudomonadota
Enterobacter
Klebsiella
Salmonella

Revision editor(s): Vandana Maddi

Signature 2

Needs review

Curated date: 2024/10/24

Curator: Vandana Maddi

Revision editor(s): Vandana Maddi

Source: Fecal microbiome of postmenopausal women

Description: Decreased Firmicutes/Bacteroidetes ratio, which is often associated with metabolic imbalance and obesity, in the high VAT group.

Abundance in Group 1: decreased abundance in High VAT group (Case/Exposed group)

NCBI Quality ControlLinks
Bacillota
Bacteroides
Ruminococcaceae sp. B_A14
Prevotella
Clostridium

Revision editor(s): Vandana Maddi

Experiment 2


Needs review

Curated date: 2024/10/24

Curator: Vandana Maddi

Revision editor(s): Vandana Maddi

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Human gut metagenome human gut microbiota,Human gut metagenome,human gut metagenome
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Low LPS levels
Group 1 name Corresponds to the case (exposed) group for case-control studies
High LPS levels
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Elevated plasma LPS levels
Group 0 sample size Number of subjects in the control (unexposed) group
23
Group 1 sample size Number of subjects in the case (exposed) group
24
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
Yes

Lab analysis

16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V6-V9

Statistical Analysis

Statistical test
T-Test
Matched on Factors on which subjects have been matched on in a case-control study
Matched on: "Age" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.Age, Matched on: "BMI" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.BMI
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
Confounders controlled for: "Smoking" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.Smoking, Confounders controlled for: "medication usge" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.medication usge

Alpha Diversity

Pielou Quantifies how equal the community is numerically
decreased
Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
decreased
Simpson Estimator of species richness and species evenness: more weight on species evenness
decreased
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
decreased
Richness Number of species
decreased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
decreased

Signature 1

Needs review

Curated date: 2024/10/24

Curator: Vandana Maddi

Revision editor(s): Vandana Maddi

Source: Gut microbiome

Description: Elevated Shigella, a genus associated with gut infections and endotoxemia, in participants with high LPS levels and adiposity.

Abundance in Group 1: increased abundance in High LPS levels

NCBI Quality ControlLinks
Shigella boydii
Salmonella
Klebsiella pneumoniae

Revision editor(s): Vandana Maddi

Signature 2

Needs review

Curated date: 2024/10/24

Curator: Vandana Maddi

Revision editor(s): Vandana Maddi

Source: Gut microbiome

Description: Reduced levels of Alistipes, a gut microbe linked to anti-inflammatory properties, in the high LPS group.

Abundance in Group 1: decreased abundance in High LPS levels

NCBI Quality ControlLinks
Faecalibacterium
Alistipes
Bacteroides uniformis

Revision editor(s): Vandana Maddi

Experiment 3


Needs review

Curated date: 2024/10/24

Curator: Vandana Maddi

Revision editor(s): Vandana Maddi

Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces , Large intestine Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces,Intestinum crassum,Large intestine,large intestine
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
IGA glomerulonephritis Berger Disease,Berger's Disease,berger's disease,berger's IgA or IgG nephropathy,focal glomerulonephritis,Glomerulonephritis, IGA,IgA glomerulonephritis,IGA Nephropathy,IgA nephropathy,Immunoglobulin A Nephropathy,Nephropathy, IGA,primary IgA nephropathy,segmental glomerulonephritis,IGA glomerulonephritis,iGA glomerulonephritis
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Low IgA response
Group 1 name Corresponds to the case (exposed) group for case-control studies
High IgA response
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Elevated anti-LPS IgA levels

Lab analysis

16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V9

Statistical Analysis

Statistical test
Pearson Correlation
Matched on Factors on which subjects have been matched on in a case-control study
Matched on: "BMI" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.BMI
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
diet, Confounders controlled for: "antibiotic use" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.antibiotic use

Alpha Diversity

Pielou Quantifies how equal the community is numerically
increased
Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Chao1 Abundance-based estimator of species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
increased
Richness Number of species
increased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
increased

Signature 1

Needs review

Curated date: 2024/10/24

Curator: Vandana Maddi

Revision editor(s): Vandana Maddi

Source: Gut microbiome

Description: Increased Escherichia coli, a gram-negative bacterium linked to inflammation, in the high IgA response group, indicating heightened immune activation.

Abundance in Group 1: increased abundance in High IgA response

NCBI Quality ControlLinks
Escherichia coli
Klebsiella oxytoca
Shigella flexneri

Revision editor(s): Vandana Maddi

Signature 2

Needs review

Curated date: 2024/10/24

Curator: Vandana Maddi

Revision editor(s): Vandana Maddi

Source: Gut microbiome

Description: Decreased abundance of Lachnospiraceae, a family associated with short-chain fatty acid production and gut health, in the high IgA group.

Abundance in Group 1: decreased abundance in High IgA response

NCBI Quality ControlLinks
Lachnospiraceae
Blautia
Coprococcus

Revision editor(s): Vandana Maddi

Experiment 4


Needs review

Curated date: 2024/10/24

Curator: Vandana Maddi

Revision editor(s): Vandana Maddi

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Eating behaviour Eating behaviour,eating behaviour
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Low HEI group
Group 1 name Corresponds to the case (exposed) group for case-control studies
High HEI group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
HEI score ≥ 70
Group 0 sample size Number of subjects in the control (unexposed) group
25
Group 1 sample size Number of subjects in the case (exposed) group
25

Lab analysis

Statistical Analysis

Statistical test
Mann-Whitney (Wilcoxon)
Matched on Factors on which subjects have been matched on in a case-control study
age, Matched on: "BMI" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.BMI
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
physical activity, Confounders controlled for: "smoking" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.smoking

Alpha Diversity

Pielou Quantifies how equal the community is numerically
increased
Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Chao1 Abundance-based estimator of species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
increased
Richness Number of species
increased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
increased

Signature 1

Needs review

Curated date: 2024/10/24

Curator: Vandana Maddi

Revision editor(s): Vandana Maddi

Source: Plasma microbiome

Description: Elevated Veillonella atypica, a bacterium linked to systemic inflammation and gut dysbiosis, in individuals with high LBP concentrations.

Abundance in Group 1: increased abundance in High HEI group

NCBI Quality ControlLinks
Veillonella atypica
Streptococcus
Megasphaera

Revision editor(s): Vandana Maddi

Signature 2

Needs review

Curated date: 2024/10/24

Curator: Vandana Maddi

Revision editor(s): Vandana Maddi

Source: Plasma microbiome

Description: Reduced Barnesiella, a beneficial gut microbe, in the high LBP group, suggesting an imbalance in microbial composition.

Abundance in Group 1: decreased abundance in High HEI group

NCBI Quality ControlLinks
Barnesiella
Bifidobacterium
Akkermansia

Revision editor(s): Vandana Maddi

Experiment 5


Needs review

Curated date: 2024/10/24

Curator: Vandana Maddi

Revision editor(s): Vandana Maddi

Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Plasma Cell Count PLSTCE,Total Plasma Cells,Plasma Cell Count,plasma Cell Count
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Low Plasma LBP group
Group 1 name Corresponds to the case (exposed) group for case-control studies
High Plasma LBP group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Elevated plasma LBP concentration
Group 0 sample size Number of subjects in the control (unexposed) group
23
Group 1 sample size Number of subjects in the case (exposed) group
24

Lab analysis

Statistical Analysis

Statistical test
Pearson Correlation
Matched on Factors on which subjects have been matched on in a case-control study
age, Matched on: "total body fat" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.total body fat
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
diet, Confounders controlled for: "medication use" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.medication use

Alpha Diversity

Pielou Quantifies how equal the community is numerically
decreased
Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
decreased
Simpson Estimator of species richness and species evenness: more weight on species evenness
decreased
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
decreased
Richness Number of species
decreased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
decreased

Signature 1

Needs review

Curated date: 2024/10/24

Curator: Vandana Maddi

Revision editor(s): Vandana Maddi

Source: Gut microbiome

Description: Increased Prevotella, a genus associated with carbohydrate-rich diets, in individuals with lower Healthy Eating Index (HEI) scores, indicative of poor diet quality.

Abundance in Group 1: increased abundance in High Plasma LBP group

NCBI Quality ControlLinks
Prevotellaceae
Dialister
Parabacteroides

Revision editor(s): Vandana Maddi

Signature 2

Needs review

Curated date: 2024/10/24

Curator: Vandana Maddi

Revision editor(s): Vandana Maddi

Source: Gut microbiome

Description: Decreased Bacteroides, a genus associated with fiber-rich diets, in individuals with lower HEI scores, reflecting poorer diet quality.

Abundance in Group 1: decreased abundance in High Plasma LBP group

NCBI Quality ControlLinks
Faecalibacterium prausnitzii
Clostridium
Bacteroides

Revision editor(s): Vandana Maddi