Shifts in microbial diversity, composition, and functionality in the gut and genital microbiome during a natural SIV infection in vervet monkeys/Experiment 1
From BugSigDB
Needs review
Subjects
- Location of subjects
- South Africa
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Chlorocebus pygerythrus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Simian immunodeficiency virus infection Simian immunodeficiency virus caused disease or disorder,Simian immunodeficiency virus disease or disorder,simian immunodeficiency virus infection,Simian immunodeficiency virus infectious disease,SIV,Simian immunodeficiency virus infection
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Simian Immunodeficiency Virus (SIV) negative
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Simian Immunodeficiency Virus (SIV) positive
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- SIV positive refers to vervet monkeys that are infected with Simian Immunodeficiency Virus (SIV)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 11
- Group 1 sample size Number of subjects in the case (exposed) group
- 33
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, geographic area, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- increased
Signature 1
Needs review
Source: Fig. 3C
Description: Differential abundance of microbiota between SIV infected and uninfected in fecal samples.
Abundance in Group 1: increased abundance in Simian Immunodeficiency Virus (SIV) positive
Revision editor(s): KateRasheed
Signature 2
Needs review
Source: Fig. 3C
Description: Differential abundance of microbiota between SIV infected and uninfected in fecal samples.
Abundance in Group 1: decreased abundance in Simian Immunodeficiency Virus (SIV) positive
| NCBI | Quality Control | Links |
|---|---|---|
| Succinivibrio |
Revision editor(s): KateRasheed
