A signature of Prevotella copri and Faecalibacterium prausnitzii depletion, and a link with bacterial glutamate degradation in the Kenyan colorectal cancer patients/Experiment 1

From BugSigDB


Needs review

Curated date: 2024/10/29

Curator: Ifeanyisam

Revision editor(s): Ifeanyisam

Subjects

Location of subjects
Kenya
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Colon Hindgut,Large bowel,Posterior intestine,Colon,colon
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Colorectal cancer cancer of colorectum,cancer of large bowel,cancer of large intestine,cancer of the large bowel,colon cancer,colorectal cancer,colorectum cancer,CRC,large intestine cancer,malignant colorectal neoplasm,malignant colorectal tumor,malignant colorectum neoplasm,malignant large bowel neoplasm,malignant large bowel tumor,malignant large intestine neoplasm,malignant large intestine tumor,malignant neoplasm of colorectum,malignant neoplasm of large bowel,malignant neoplasm of large intestine,malignant neoplasm of the large bowel,malignant neoplasm of the large intestine,malignant tumor of large bowel,malignant tumor of large intestine,malignant tumor of the large bowel,malignant tumor of the large intestine,Colorectal cancer
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy Control
Group 1 name Corresponds to the case (exposed) group for case-control studies
CRC Patients
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
patients with Colorectal Cancer
Group 0 sample size Number of subjects in the control (unexposed) group
18
Group 1 sample size Number of subjects in the case (exposed) group
18
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
4 weeks

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2.0

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Needs review

Curated date: 2024/10/29

Curator: Ifeanyisam

Revision editor(s): Ifeanyisam

Source: Figure 2

Description: Bar graph of LEfSe analysis CRC vs. healthy controls

Abundance in Group 1: increased abundance in CRC Patients

NCBI Quality ControlLinks
Mycoplasma
Mycoplasmatales
Campylobacter ureolyticus
Mycoplasmataceae
Peptostreptococcus anaerobius
Rhodospirillales
Mogibacterium
Anaerococcus
Peptoniphilus
Lactobacillus iners
Mobiluncus
Solobacterium moorei
Helicobacteraceae
Flexispira
Moryella indoligenes
Moryella
1_681_68
Clostridium
Schwartzia
Sporobacterium sp. WAL 1855D
Veillonella dispar
Veillonella parvula
Peptostreptococcus
p_75_a5p_75_a5
Staphylococcaceae
Staphylococcus
Parvimonas
Bacillales
Tissierellaceae
Porphyromonas
Prevotella nigrescens
Fusobacterium
Bacteroides fragilis

Revision editor(s): Ifeanyisam

Signature 2

Needs review

Curated date: 2024/10/29

Curator: Ifeanyisam

Revision editor(s): Ifeanyisam

Source: Figure 2

Description: Bar graph of LEfSe analysis CRC vs. healthy controls.

Abundance in Group 1: decreased abundance in CRC Patients

NCBI Quality ControlLinks
Actinomycetales
Alcaligenaceae
Alphaproteobacteria
Bacteroides
Bacteroides eggerthii
Burkholderiales
Collinsella
Collinsella aerofaciens
Coriobacteriales
Coriobacteriia
Cyanobacteriota
Dorea formicigenerans
Erysipelotrichaceae
Eubacterium
Faecalibacterium
Faecalibacterium prausnitzii
Haemophilus parainfluenzae
Holdemanella biformis
Parabacteroides
Pasteurellaceae
Pasteurellales
Roseburia
Roseburia faecis
Segatella copri
Sutterella
4COd_24COd_2
YS2YS2
[Ruminococcus] lactaris

Revision editor(s): Ifeanyisam