The Parkinson's disease drug entacapone disrupts gut microbiome homoeostasis via iron sequestration/Experiment 2
From BugSigDB
Needs review
Subjects
- Location of subjects
- Austria
- United Kingdom
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to drug drug resistance,drug susceptibility/resistance,Response to drug,response to drug
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Loxapine Succinate High (LOX-High), Entacapone Concentration(ENT-High) and Entacapone Low(ENT-Low) after 24hours of incubation
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Loxapine Succinate Low Concentration(LOX-Low) after 24 hrs of incubation
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Loxapine Succinate Low Concentration(LOX-Low) after 24 hrs of incubation refers to the drug concentration given to the six healthy adult individuals after twenty-four hours of incubation.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 9
- Group 1 sample size Number of subjects in the case (exposed) group
- 3
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Signature 1
Needs review
Source: Supplementary Table 5
Description: Differential abundance of taxa between the drug-amendment experiments(ENT-High, ENT-Low, LOX-High and LOX-Low) after 24hrs of incubation.
Abundance in Group 1: increased abundance in Loxapine Succinate Low Concentration(LOX-Low) after 24 hrs of incubation
Revision editor(s): KateRasheed
Signature 2
Needs review
Source: Supplementary Table 5
Description: Differential abundance of taxa between the drug-amendment experiments(ENT-High, ENT-Low, LOX-High and LOX-Low) after 24hrs of incubation.
Abundance in Group 1: decreased abundance in Loxapine Succinate Low Concentration(LOX-Low) after 24 hrs of incubation
Revision editor(s): KateRasheed
