Intestinal permeability before and after albendazole treatment in low and high socioeconomic status schoolchildren in Makassar, Indonesia
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Authors
Amaruddin AI, Koopman JPR, Muhammad M, Lenaerts K, van Eijk HMH, Brienen EAT, Geelen AR, van Lieshout L, Wahyuni S, Kuijper EJ, Zwittink RD, Hamid F, Sartono E, Yazdanbakhsh M
Journal
Scientific reports
Year
2022
Intestinal helminths are highly prevalent in low-SES children and could contribute to poor health outcomes either directly or via alteration of the gut microbiome and gut barrier function. We analysed parasitic infections and gut microbiota composition in 325 children attending high- and low-SES schools in Makassar, Indonesia before and after albendazole treatment. Lactulose/Mannitol Ratio (LMR, a marker of gut permeability); I-FABP (a surrogate marker of intestinal damage) as well as inflammatory markers (LBP) were measured. Helminth infections were highly prevalent (65.6%) in low-SES children. LMR and I-FABP levels were higher in low-SES children (geomean (95%CI): 4.03 (3.67-4.42) vs. 3.22 (2.91-3.57); p. adj < 0.001; and 1.57 (1.42-1.74) vs. 1.25 (1.13-1.38); p. adj = 0.02, respectively) while LBP levels were lower compared to the high-SES (19.39 (17.09-22.01) vs. 22.74 (20.07-26.12); p.adj = 0.01). Albendazole reduced helminth infections in low-SES and also decreased LMR with 11% reduction but only in helminth-uninfected children (estimated treatment effect: 0.89; p.adj = 0.01). Following treatment, I-FABP decreased in high- (0.91, p.adj < 0.001) but increased (1.12, p.adj = 0.004) in low-SES children. Albendazole did not alter the levels of LBP. Microbiota analysis showed no contribution from specific bacterial-taxa to the changes observed. Intestinal permeability and epithelial damage are higher while peripheral blood inflammatory marker is lower in children of low-SES in Indonesia. Furthermore, treatment decreased LMR in helminth-uninfected only.
Experiment 1
Needs review
Subjects
- Location of subjects
- Indonesia
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Low-SES helminth-uninfected group (before treatment)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Low-SES helminth-uninfected group (after treatment)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Stool samples collected four (4) weeks after albendazole treatment from children of low socioeconomic status (SES) who were uninfected with helminths.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 29
- Group 1 sample size Number of subjects in the case (exposed) group
- 29
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Needs review
Source: Figure 3B
Description: Differential abundance analysis by DESeq2 indicating increased relative abundance and decreased relative abundance after treatment with albendazole in Low-SES helminth-uninfected children.
Abundance in Group 1: increased abundance in Low-SES helminth-uninfected group (after treatment)
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacteriaceae | ||
| Bifidobacterium | ||
| Lactobacillus | ||
| Streptococcus | ||
| Subdoligranulum | ||
| Clostridiales (Family XIII AD3011 group)Clostridiales (Family XIII AD3011 group) |
Revision editor(s): Aleru Divine
Signature 2
Needs review
Source: Figure 3B
Description: Differential abundance analysis by DESeq2 indicating increased relative abundance and decreased relative abundance after treatment with albendazole in Low-SES helminth-uninfected children.
Abundance in Group 1: decreased abundance in Low-SES helminth-uninfected group (after treatment)
| NCBI | Quality Control | Links |
|---|---|---|
| Dialister | ||
| Faecalibacterium | ||
| Prevotella | ||
| Succinivibrio | ||
| unclassified Oscillospiraceae | ||
| Rikenellaceae RC9Rikenellaceae RC9 |
Revision editor(s): Aleru Divine
Experiment 2
Needs review
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- High-SES group (before treatment)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- High-SES group (after treatment)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Stool samples collected four (4) weeks after albendazole treatment from children of high socioeconomic status (SES).
- Group 0 sample size Number of subjects in the control (unexposed) group
- 66
- Group 1 sample size Number of subjects in the case (exposed) group
- 66
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Needs review
Source: Figure 3B
Description: Differential abundance analysis by DESeq2 indicating increased relative abundance and decreased relative abundance after treatment with albendazole in High-SES children.
Abundance in Group 1: increased abundance in High-SES group (after treatment)
| NCBI | Quality Control | Links |
|---|---|---|
| Clostridiales (Family XIII AD3011 group)Clostridiales (Family XIII AD3011 group) | ||
| Streptococcus | ||
| Lactobacillus |
Revision editor(s): Aleru Divine
Signature 2
Needs review
Source: Figure 3B
Description: Differential abundance analysis by DESeq2 indicating increased relative abundance and decreased relative abundance after treatment with albendazole in High-SES children.
Abundance in Group 1: decreased abundance in High-SES group (after treatment)
| NCBI | Quality Control | Links |
|---|---|---|
| Prevotella | ||
| Faecalibacterium |
Revision editor(s): Aleru Divine
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