The tongue microbiome in healthy subjects and patients with intra-oral halitosis

Study information

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

28875948

DOI Digital object identifier for electronic documents.

10.1088/1752-7163/aa7c24

URI

Authors

Seerangaiyan K, van Winkelhoff AJ, Harmsen HJM, Rossen JWA, Winkel EG

Journal

Journal of breath research

Year

2017

Intra-oral halitosis (IOH) is an unpleasant odor emanating from the oral cavity. It is thought that the microbiota of the dorsal tongue coating plays a crucial role in this condition. The aim of the study was to investigate the composition of the tongue microbiome in subjects with and without IOH. A total of 26 subjects, 16 IOH patients and 10 healthy subjects were recruited based on their organoleptic score and volatile sulfur compound (VSC) measurements. The composition of the tongue microbiome was studied using the 16s amplicon sequencing of the V3-V4 hyper variable region with an Illumina MiSeq. The sequenced data were analyzed using QIIME, and the sequences obtained were distributed across 7 phyla, 27 genera and 825 operational taxonomic units (OTUs). At a higher taxon level, TM7 was associated with IOH patients whereas Gemellaceae was significantly abundant in the healthy subjects. At OTU level, we found several significant OTUs that differentiated the IOH patients from the controls. These included Aggregatibacter (OTU id 4335776), Aggregatibacter segnis (A. segnis), Campylobacter, Capnocytophaga, Clostridiales, Dialister, Leptotrichia, Parvimonas, Peptostreptococcus, Peptococcus, Prevotella, Selenomonas, SR1, Tannerella, TM7-3 and Treponema in the IOH group. In the control group, Aggregatibacter (OTU id 4363066), Haemophilus, Haemophilus parainfluenza (H. parainfluenza), Moryella, Oribacterium, Prevotella, several Streptococcus, Rothia dentocariosa (R. dentocariosa) and OTU from Gemellaceae were significantly abundant. Based on our observation, it was concluded that the bacterial qualitative composition of the IOH and the control group was almost the same, except for the few above-mentioned bacterial species and genera.

Experiment 1

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Claregrieve1, Davvve, Victoria

Subjects

Location of subjects: Netherlands

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Superior surface of tongue Dorsal surface of tongue,Superior surface of tongue,superior surface of tongue

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Halitosis oral halitosis,Halitosis,halitosis

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Control

Group 1 name Corresponds to the case (exposed) group for case-control studies: Oral Halitosis

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: IOH patient group was selected based on an organoleptic score of >= 2 from the mouth and nose =< 1, having a VSC level > 160 ppb, and H2S > 4 nmol/ L (96 ppb) and CH3SH > 0.5 nmol/L (12 ppb) and a DPSI of =< 2

Group 0 sample size Number of subjects in the control (unexposed) group: 5

Group 1 sample size Number of subjects in the case (exposed) group: 10

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 3 months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: raw counts

Statistical test: DESeq2

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Signature 1

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Phyu Han

Revision editor(s): WikiWorks

Source: Figure 4

Description: Significant differentially abundant OTU of intr-aoral halitosis and Control

Abundance in Group 1: increased abundance in Oral Halitosis

NCBI	Quality Control	Links
Aggregatibacter segnis
Campylobacter
Tannerella
Dialister
Peptostreptococcus
Selenomonas
Leptotrichia
Peptococcus
Eubacteriales
Parvimonas
Treponema
Capnocytophaga
Aggregatibacter
Prevotella