Air pollution during the winter period and respiratory tract microbial imbalance in a healthy young population in Northeastern China

Study information

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

31126003

DOI Digital object identifier for electronic documents.

10.1016/j.envpol.2018.12.083

URI

Authors

Li X, Sun Y, An Y, Wang R, Lin H, Liu M, Li S, Ma M, Xiao C

Journal

Environmental pollution (Barking, Essex : 1987)

Year

2019

Keywords:

Air pollution, Airborne particulate matter, Coal burning, Oropharyngeal microbiota, Respiratory tract diseases

In order to investigate the relationship between air pollution and the respiratory tract microbiota, 114 healthy volunteers aged 18-21 years were selected during the winter heating period in Northeast China; 35 from a lightly polluted region (group A), 40 from a moderately polluted region (group B) and 39 from a heavily polluted region (group C). Microbial genome DNA was extracted from throat swab samples to study the oral flora composition of the volunteers by amplifying and sequencing the V3 regions of prokaryotic 16S rRNA. Lung function tests were also performed. The relative abundance of Bacteroidetes and Fusobacteria were significantly lower and Firmicutes Proteonacteria and Actinobacteria higher in participants from polluted regions. Within bacteria classes, Bacterioida abundance was lower and Clostridia abundance higher in polluted areas, which was also reflected in the order of abundance. In samples from region C, the abundance of Prevotellaceae, Veillonellaceae, Porphyromonadaceae, Fusobacteriaceae Paraprevollaceae and Flavobacteriaceae were lowest among the 3 regions studied, whereas the abundance of Lachnospiraceae and Ruminococcaceae were the highest. From group A to group C, the relative class abundances of Prevotella, Veillonella, Fusobacterium, Camphylobacter and Capnocytophaga Porphyromonas, Peptostreptococcus and Moraxella became lower in polluted areas. Pulmonary function correlated with air pollution and the oropharyngeal microbiota differed within regions of high, medium and low air pollution. Thus, during the winter heating period in Northeast China, the imbalance of the oropharyngeal microbiota might be caused by air pollution and is likely associated with impairment of lung function in young people.

Experiment 1

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Lwaldron, Davvve, Victoria

Subjects

Location of subjects

China

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Throat Gula,Throat,throat

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Air pollution air pollution,Air pollution

Group 0 name Corresponds to the control (unexposed) group for case-control studies

lightly poluuted region (A)

Group 1 name Corresponds to the case (exposed) group for case-control studies

moderately polluted region (B)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

paticipants lived in any of those regions for more than 2 years. region A: (lightly polluted region of PM 2.5), region B: (moderately polluted region of PM 2.5) and region C: (heavily polluted region of PM 2.5)

Group 0 sample size Number of subjects in the control (unexposed) group

35

Group 1 sample size Number of subjects in the case (exposed) group

40

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)

3 months

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V3

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

No

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment

age, body height, body weight, vital capacity, sex

Signature 1

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 3, 4, 5, 6

Description: Abundance analysis of the dominant bacterial taxa of A (lightly polluted region of PM 2.5), B (moderately polluted region of PM 2.5) and C (heavily polluted region of PM 2.5)

Abundance in Group 1: increased abundance in moderately polluted region (B)

NCBI	Quality Control	Links
Bacillota
Actinomycetota
Clostridia
Actinomycetes
Eubacteriales
Actinomycetales
Lachnospiraceae
Micrococcaceae
Actinomycetaceae
Oscillospiraceae
Rothia
Oribacterium
Neisseria
Actinomyces

Revision editor(s): WikiWorks

Signature 2

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): Merit, WikiWorks

Source: Figure 3, 4, 5, 6

Description: Abundance analysis of the dominant bacterial taxa of A (lightly polluted region of PM 2.5), B (moderately polluted region of PM 2.5) and C (heavily polluted region of PM 2.5)

Abundance in Group 1: decreased abundance in moderately polluted region (B)

NCBI	Quality Control	Links
Bacteroidales
Bacteroidia
Bacteroidota
Flavobacteriales
Fusobacteriaceae
Fusobacteriales
Fusobacteriia
Fusobacteriota
Fusobacterium
Moraxella
Moraxellaceae
Peptostreptococcus
Porphyromonadaceae
Porphyromonas
Prevotella
Prevotellaceae
Pseudomonadales
Veillonella
Veillonellaceae

Revision editor(s): Merit, WikiWorks

Experiment 2

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees, Joan Chuks, Victoria

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies

heavily polluted region (C)

Group 1 sample size Number of subjects in the case (exposed) group

39

Lab analysis

Statistical Analysis

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment

age, body height, body weight, vital capacity, sex, Confounders controlled for: "peak expiratory flow" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.peak expiratory flow

Signature 1

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 3, 4, 5, 6

Description: Abundance analysis of the dominant bacterial taxa of A (lightly polluted region of PM 2.5), B (moderately polluted region of PM 2.5) and C (heavily polluted region of PM 2.5)

Abundance in Group 1: increased abundance in heavily polluted region (C)

NCBI	Quality Control	Links
Bacillota
Pseudomonadota
Actinomycetota
Clostridia
Oscillospiraceae
Gammaproteobacteria
Alphaproteobacteria
Eubacteriales
Lachnospiraceae

Revision editor(s): WikiWorks

Signature 2

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): Merit, WikiWorks

Source: Figure 3, 4, 5, 6

Description: Abundance analysis of the dominant bacterial taxa of A (lightly polluted region of PM 2.5), B (moderately polluted region of PM 2.5) and C (heavily polluted region of PM 2.5)

Abundance in Group 1: decreased abundance in heavily polluted region (C)

NCBI	Quality Control	Links
Bacteroidales
Bacteroidia
Bacteroidota
Campylobacter
Campylobacterales
Capnocytophaga
Flavobacteriaceae
Flavobacteriales
Flavobacteriia
Fusobacteriaceae
Fusobacteriales
Fusobacteriia
Fusobacteriota
Fusobacterium
Moraxella
Moraxellaceae
Neisseriales
Peptostreptococcus
Porphyromonadaceae
Porphyromonas
Prevotella
Prevotellaceae
Veillonella
Veillonellaceae

Revision editor(s): Merit, WikiWorks

Experiment 3

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees, Scholastica, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies

moderately poluuted region (B)

Group 0 sample size Number of subjects in the control (unexposed) group

40

Lab analysis

Statistical Analysis

Signature 1

EditHistoryDelete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 3, 4, 5, 6

Description: Abundance analysis of the dominant bacterial taxa of A (lightly polluted region of PM 2.5), B (moderately polluted region of PM 2.5) and C (heavily polluted region of PM 2.5)

Abundance in Group 1: increased abundance in heavily polluted region (C)

NCBI	Quality Control	Links
Pseudomonadota
Gammaproteobacteria
Alphaproteobacteria
Pseudomonadales

Revision editor(s): WikiWorks

Signature 2

EditHistoryDelete

Flag for review

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 3, 4, 5, 6

Description: Abundance analysis of the dominant bacterial taxa of A (lightly polluted region of PM 2.5), B (moderately polluted region of PM 2.5) and C (heavily polluted region of PM 2.5)

Abundance in Group 1: decreased abundance in heavily polluted region (C)

NCBI	Quality Control	Links
Fusobacteriota
Actinomycetes
Fusobacteriia
Actinomycetales
Fusobacteriales
Neisseriales
Campylobacterales
Micrococcaceae
Fusobacteriaceae
Neisseriaceae
Leptotrichiaceae
Actinomycetaceae
Rothia
Fusobacterium
Oribacterium
Actinomyces
Campylobacter
Neisseria

Revision editor(s): WikiWorks