The Placental Microbiota Is Altered among Subjects with Gestational Diabetes Mellitus: A Pilot Study

Study information

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

28932201

DOI Digital object identifier for electronic documents.

10.3389/fphys.2017.00675

URI

Authors

Zheng J, Xiao X, Zhang Q, Mao L, Yu M, Xu J, Wang T

Journal

Frontiers in physiology

Year

2017

Keywords:

16S rRNA gene, clinical characteristics, gestational diabetes mellitus, microbiota, placenta

Gestational diabetes mellitus (GDM) has significant implications for the future health of the mother and child. However, the associations between human placental microbiota and GDM are poorly understood. We aimed to profile the placental microbiota of GDM and further define whether or not certain placental microbiota taxon correlates with specific clinical characteristics. Placenta were collected from GDM women and women with normal pregnancies (n = 10, in each group) consecutively recruited at Peking Union Medical College Hospital. The anthropometric parameters of mother and infant, and cord blood hormones, including insulin, leptin and insulin-like growth factor-1 (IGF-1) were measured. Bacterial genomic DNA was isolated using magnetic beads and the human placental microbiota was analyzed using the Illumina MiSeq Sequencing System based on the V3-V4 hypervariable regions of the 16S rRNA gene. It showed there was no statistical difference in the clinical characteristics of mothers and infants, such as BMI at the beginning of pregnancy and gestational weight gain (GWG), birth weight, and cord blood hormones, including insulin, leptin and IGF-1. We found that the placental microbiota is composed of four dominant phyla from Proteobacteria (the most abundant), Bacteroidetes, Actinobacteria and Firmicutes, with the proportion of Proteobacteria increased, and Bacteroidetes and Firmicutes were decreased of women with GDM. Further analyses suggested that bacterial taxonomic composition of placentas from the phylum level down to the bacteria level, differed significantly between women with GDM and non-GDM women with normal pregnancies. Regression analysis showed a cluster of key operational taxonomic units (OTUs), phyla and genera were significantly correlated with GWG during pregnancy of mothers, and cord blood insulin, IGF-1 and leptin concentrations. In conclusion, our novel study showed that a distinct placental microbiota profile is present in GDM, and is associated with clinical characteristics of mothers and infants. This study contributes to the theoretical foundation on the potential relationship between placental microbiota and GDM.

Experiment 1

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Uterus Uterus,uterus

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Gestational diabetes diabetes in pregnancy,GDM,gestational diabetes,gestational diabetes mellitus,maternal gestational diabetes mellitus,Gestational diabetes

Group 0 name Corresponds to the control (unexposed) group for case-control studies: controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: Gestational diabetes mellitus

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: GDM was diagnosed when the fasting plasma glucose ≥5.1 mmol/L or 1 h post-OGTT glycemia ≥10.0 mmol/L or 2 h post-OGTT glycemia ≥8.5 mmol/L, according to the criteria set by International Association of Diabetes and Pregnancy Study Groups (IADPSG)

Group 0 sample size Number of subjects in the control (unexposed) group: 10

Group 1 sample size Number of subjects in the case (exposed) group: 10

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 9 months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Matched on Factors on which subjects have been matched on in a case-control study: age, body mass index

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Richness Number of species: unchanged

Signature 1

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Table 2+ Figure 4+ Supplemental Table S2

Description: Phlotypes in placental microbiota significantly different between GDM and NDM groups

Abundance in Group 1: increased abundance in Gestational diabetes mellitus

NCBI	Quality Control	Links
Pseudomonadota
Thauera
Burkholderiales
Hyphomicrobiales
Alcaligenaceae

Revision editor(s): WikiWorks

Signature 2

Edit History Delete

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Table 2+ Figure 4+ Supplemental Table S2

Description: Phlotypes in placental microbiota significantly different between GDM and NDM groups

Abundance in Group 1: decreased abundance in Gestational diabetes mellitus

NCBI	Quality Control	Links
Bacteroidota
Armatimonadota
Brevibacillus
Sulfuricella
Lactobacillus
Dyella
Bacteroidales
Erysipelotrichales
Holophagales
Hydrogenophilales
Ignavibacteriales
Chlorobiota
Bacteroidia
Geothrix
Ignavibacteria
Lactobacillaceae
Hydrogenophilaceae
Paenibacillaceae

Revision editor(s): WikiWorks