The Placental Microbiota Is Altered among Subjects with Gestational Diabetes Mellitus: A Pilot Study/Experiment 1

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Uterus Uterus,uterus
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Gestational diabetes diabetes in pregnancy,GDM,gestational diabetes,gestational diabetes mellitus,maternal gestational diabetes mellitus,Gestational diabetes
Group 0 name Corresponds to the control (unexposed) group for case-control studies
controls
Group 1 name Corresponds to the case (exposed) group for case-control studies
Gestational diabetes mellitus
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
GDM was diagnosed when the fasting plasma glucose ≥5.1 mmol/L or 1 h post-OGTT glycemia ≥10.0 mmol/L or 2 h post-OGTT glycemia ≥8.5 mmol/L, according to the criteria set by International Association of Diabetes and Pregnancy Study Groups (IADPSG)
Group 0 sample size Number of subjects in the control (unexposed) group
10
Group 1 sample size Number of subjects in the case (exposed) group
10
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
9 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
Matched on Factors on which subjects have been matched on in a case-control study
age, body mass index

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Table 2+ Figure 4+ Supplemental Table S2

Description: Phlotypes in placental microbiota significantly different between GDM and NDM groups

Abundance in Group 1: increased abundance in Gestational diabetes mellitus

NCBI Quality ControlLinks
Pseudomonadota
Thauera
Burkholderiales
Hyphomicrobiales
Alcaligenaceae

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Table 2+ Figure 4+ Supplemental Table S2

Description: Phlotypes in placental microbiota significantly different between GDM and NDM groups

Abundance in Group 1: decreased abundance in Gestational diabetes mellitus

NCBI Quality ControlLinks
Bacteroidota
Armatimonadota
Brevibacillus
Sulfuricella
Lactobacillus
Dyella
Bacteroidales
Erysipelotrichales
Holophagales
Hydrogenophilales
Ignavibacteriales
Chlorobiota
Bacteroidia
Geothrix
Ignavibacteria
Lactobacillaceae
Hydrogenophilaceae
Paenibacillaceae

Revision editor(s): WikiWorks