Oral Microbiota and Risk for Esophageal Squamous Cell Carcinoma in a High-Risk Area of China

From BugSigDB
Needs review
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Chen X, Winckler B, Lu M, Cheng H, Yuan Z, Yang Y, Jin L, Ye W
Journal
PloS one
Year
2015
Poor oral health has been linked with an increased risk of esophageal squamous cell carcinoma (ESCC). We investigated whether alteration of oral microbiota is associated with ESCC risk. Fasting saliva samples were collected from 87 incident and histopathologicallly diagnosed ESCC cases, 63 subjects with dysplasia and 85 healthy controls. All subjects were also interviewed with a questionnaire. V3-V4 region of 16S rRNA was amplified and sequenced by 454-pyrosequencing platform. Carriage of each genus was compared by means of multivariate-adjusted odds ratios derived from logistic regression model. Relative abundance was compared using Metastats method. Beta diversity was estimated using Unifrac and weighted Unifrac distances. Principal coordinate analysis (PCoA) was applied to ordinate dissimilarity matrices. Multinomial logistic regression was used to compare the coordinates between different groups. ESCC subjects had an overall decreased microbial diversity compared to control and dysplasia subjects (P<0.001). Decreased carriage of genera Lautropia, Bulleidia, Catonella, Corynebacterium, Moryella, Peptococcus and Cardiobacterium were found in ESCC subjects compared to non-ESCC subjects. Multinomial logistic regression analyses on PCoA coordinates also revealed that ESCC subjects had significantly different levels for several coordinates compared to non-ESCC subjects. In conclusion, we observed a correlation between altered salivary bacterial microbiota and ESCC risk. The results of our study on the saliva microbiome are of particular interest as it reflects the shift in microbial communities. Further studies are warranted to verify this finding, and if being verified, to explore the underlying mechanisms.

Experiment 1


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Rimsha, WikiWorks

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled (if applicable)
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
esophageal cancer Aerodigestive tract cancer, susceptibility to,Ca lower third oesophagus,Ca middle third oesophagus,cancer of esophagus,Escc, susceptibility to,esophageal cancer,esophageal squamous cell carcinoma, susceptibility to,esophagus cancer,gastric cardia adenocarcinoma, susceptibility to,malignant esophageal neoplasm,malignant esophageal tumor,malignant esophagus neoplasm,malignant esophagus tumor,malignant neoplasm of distal third of esophagus,malignant neoplasm of esophagus,malignant neoplasm of lower third of oesophagus,malignant neoplasm of middle third of oesophagus,malignant neoplasm of proximal third of esophagus,malignant neoplasm of the esophagus,malignant neoplasm of upper third esophagus,malignant tumor of abdominal esophagus,malignant tumor of distal third of esophagus,malignant tumor of esophagus,malignant tumor of proximal third of esophagus,malignant tumor of the esophagus,malignant tumor of the middle third of the esophagus
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Dysplasia
Group 1 name Corresponds to the case (exposed) group for case-control studies
Esophageal Squamous cell carcinoma (ESCC)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Histopathological confirmation, complete questionnaire with saliva sample
Group 0 sample size Number of subjects in the control (unexposed) group
63
Group 1 sample size Number of subjects in the case (exposed) group
87
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Roche454

Statistical Analysis

Statistical test
T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
Matched on Factors on which subjects have been matched on in a case-control study
age, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
unchanged

Signature 1

Needs review

Curated date: 2021/01/10

Curator: Utsav Patel

Revision editor(s): WikiWorks

Source: Fig 1, Table 2, Table 3

Description: Oral microbiota and Risk for Esophageal Squamous cell carcinoma

Abundance in Group 1: increased abundance in Esophageal Squamous cell carcinoma (ESCC)

NCBI Quality ControlLinks
Prevotella
Streptococcus
Porphyromonas
Lactobacillus

Revision editor(s): WikiWorks

Signature 2

Needs review

Curated date: 2021/01/10

Curator: Utsav Patel

Revision editor(s): WikiWorks

Source: Fig 1, Table 2, Table 3

Description: Oral microbiota and Risk for Esophageal Squamous cell carcinoma

Abundance in Group 1: decreased abundance in Esophageal Squamous cell carcinoma (ESCC)

NCBI Quality ControlLinks
Veillonella
Neisseria
Fusobacterium
Haemophilus
Rothia
Leptotrichia
Campylobacter
Aggregatibacter
Oribacterium
Atopobium
Lautropia
Bulleidia
Catonella
Corynebacterium
Peptococcus
Cardiobacterium
Herbaspirillum

Revision editor(s): WikiWorks

Experiment 2


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Rimsha, WikiWorks

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
controls
Group 0 sample size Number of subjects in the control (unexposed) group
85

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
decreased

Signature 1

Needs review

Curated date: 2021/01/10

Curator: Utsav Patel

Revision editor(s): WikiWorks

Source: Fig 1, Table 2, Table 3

Description: Oral microbiota and Risk for Esophageal Squamous cell carcinoma

Abundance in Group 1: increased abundance in Esophageal Squamous cell carcinoma (ESCC)

NCBI Quality ControlLinks
Prevotella
Streptococcus
Porphyromonas
Capnocytophaga
Elizabethkingia
Bacteroides

Revision editor(s): WikiWorks

Signature 2

Needs review

Curated date: 2021/01/10

Curator: Utsav Patel

Revision editor(s): WikiWorks

Source: Fig 1, Table 2, Table 3

Description: Oral microbiota and Risk for Esophageal Squamous cell carcinoma

Abundance in Group 1: decreased abundance in Esophageal Squamous cell carcinoma (ESCC)

NCBI Quality ControlLinks
Veillonella
Neisseria
Haemophilus
Rothia
Treponema
Megasphaera
Campylobacter
Selenomonas
Aggregatibacter
Peptostreptococcus
Oribacterium
Actinomyces
Atopobium
Lautropia
Bulleidia
Catonella
Filifactor
Corynebacterium
Acholeplasma
Butyrivibrio
Dialister
Peptococcus
Cardiobacterium

Revision editor(s): WikiWorks