Subgingival Microbial Changes During the First 3 Months of Fixed Appliance Treatment in Female Adult Patients

Study information

Needs review

study design

time series / longitudinal observational

Citation

PMID PubMed identifier for scientific articles.

30542916

DOI Digital object identifier for electronic documents.

10.1007/s00284-018-1610-1

URI

Authors

Guo R, Liu H, Li X, Yang Q, Jia L, Zheng Y, Li W

Journal

Current microbiology

Year

2019

Although periodontal diseases during fixed appliance treatment are a common issue, few studies have focused on the clinical and microbial factors associated with orthodontic appliances. Hence, we investigated changes in the subgingival microbial community and their association with periodontal changes at the early stage of fixed appliance treatment. Subgingival plaques from ten female patients with fixed appliances were obtained at three time points: before, 1 month and 3 months after the placement of the brackets (T0, T1 and T2). The 16S rRNA gene sequencing was used to analyze the microbial community of the subgingival plaque. The Plaque Index (PI) and Gingival Bleeding Index (GBI) were also recorded. The GBI significantly increased at T2, and the PI showed a temporary increase without a significant difference. The alpha diversity indices were stable. However, the beta diversity was significantly higher at T2 compared to T0 and T1. The relative abundance of core microbiomes at the genus level was relatively stable. Four periodontal pathogens at the species level, including Prevotella intermedia (Pi), Campylobacer rectus (Cr), Fusobacterium nucleatum (Fn), and Treponema denticola (Td), increased without significant differences. The subgingival microbial community affected by fixed appliance treatment might cause transient mild gingival inflammation.

Experiment 1

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Curated date: 2021/01/10

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Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Dental plaque Bacterial plaque,Plaque,Dental plaque,dental plaque

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Gingival disease disease of gingiva,disease or disorder of gingiva,disorder of gingiva,gingiva disease,gingiva disease or disorder,gingival disease,Gingival disease

Group 0 name Corresponds to the control (unexposed) group for case-control studies: before

Group 1 name Corresponds to the case (exposed) group for case-control studies: 1 month after the placement of the brackets

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: subgingial microbial changes during fixed appliance treatment

Group 0 sample size Number of subjects in the control (unexposed) group: 10

Group 1 sample size Number of subjects in the case (exposed) group: 10

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 1 month

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: Metastats

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa: unchanged

Richness Number of species: unchanged

Signature 1

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Curated date: 2021/01/10

Curator: Rimsha Azhar

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Source: Figure 5

Description: Relative abundance of microbiota that showed statistically significant differences among three different time points

Abundance in Group 1: increased abundance in 1 month after the placement of the brackets

NCBI	Quality Control	Links
Actinobacillus
Capnocytophaga

Revision editor(s): WikiWorks

Experiment 2

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Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies: 3 months after the placement of the brackets

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa: unchanged

Richness Number of species: unchanged

Signature 1

Edit History Delete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 5

Description: Relative abundance of microbiota that showed statistically significant differences among three different time points

Abundance in Group 1: increased abundance in 3 months after the placement of the brackets

NCBI	Quality Control	Links
Capnocytophaga

Revision editor(s): WikiWorks

Signature 2

Edit History Delete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): Fatima, WikiWorks

Source: Figure 5

Description: Relative abundance of microbiota that showed statistically significant differences among three different time points

Abundance in Group 1: decreased abundance in 3 months after the placement of the brackets

NCBI	Quality Control	Links
Granulicatella
Streptococcus oralis subsp. tigurinus

Revision editor(s): Fatima, WikiWorks