Melatonin prevents obesity through modulation of gut microbiota in mice
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled (if applicable)
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- normal chow diet
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- high fat diet
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Obesity is (BMI ≥ 30kg/m2)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 5
- Group 1 sample size Number of subjects in the case (exposed) group
- 5
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Source: Figure 6B + Data S1
Description: Differential abundant taxa in gut microbiota in response to melatonin treatment
Abundance in Group 1: increased abundance in high fat diet
NCBI | Links |
---|---|
Acetitomaculum | |
Prevotellaceae | |
Ruminococcus | |
Marvinbryantia | |
Alistipes | |
Blautia |
Revision editor(s): WikiWorks
Signature 2
Source: Figure 6B + Data S1
Description: Differential abundant taxa in gut microbiota in response to melatonin treatment
Abundance in Group 1: decreased abundance in high fat diet
NCBI | Links |
---|---|
Bacteroides | |
Odoribacter | |
Anaerovorax ⚠ | |
Butyricimonas | |
Christensenellaceae | |
Helicobacter | |
Christensenella ⚠ | |
Bilophila | |
Mycoplasmatota | |
Oscillibacter |
Revision editor(s): WikiWorks
Experiment 2
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled (if applicable)
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- high fat diet
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- high fat diet + melatonin
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Obesity is (BMI ≥ 30kg/m2)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 5
- Group 1 sample size Number of subjects in the case (exposed) group
- 5
Lab analysis
- Sequencing type
- 16S
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
Signature 1
Source: Figure 6B + Data S1
Description: Differential abundant taxa in gut microbiota in response to melatonin treatment
Abundance in Group 1: increased abundance in high fat diet + melatonin
NCBI | Links |
---|---|
Proteus | |
Odoribacter | |
Helicobacter | |
Anaerovorax ⚠ | |
Desulfovibrio | |
Desulfovibrionaceae | |
Butyricimonas | |
Akkermansia | |
Antricoccus |
Revision editor(s): WikiWorks
Signature 2
Source: Figure 6B + Data S1 + Figure 7
Description: Differential abundant taxa in gut microbiota in response to melatonin treatment
Abundance in Group 1: decreased abundance in high fat diet + melatonin
NCBI | Links |
---|---|
Alistipes | |
Anaerotruncus | |
Helicobacter marmotae |
Revision editor(s): WikiWorks