Melatonin prevents obesity through modulation of gut microbiota in mice
From BugSigDB
Jump to:navigation, search
Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Xu P, Wang J, Hong F, Wang S, Jin X, Xue T, Jia L, Zhai Y
Journal
Journal of pineal research
Year
2017
Keywords:
gut microbiota, inflammation, insulin resistance, liver steatosis, melatonin, obesity
Excess weight and obesity are severe public health threats worldwide. Recent evidence demonstrates that gut microbiota dysbiosis contributes to obesity and its comorbidities. The body weight-reducing and energy balancing effects of melatonin have been reported in several studies, but to date, no investigations toward examining whether the beneficial effects of melatonin are associated with gut microbiota have been carried out. In this study, we show that melatonin reduces body weight, liver steatosis, and low-grade inflammation as well as improving insulin resistance in high fat diet (HFD)-fed mice. High-throughput pyrosequencing of the 16S rRNA demonstrated that melatonin treatment significantly changed the composition of the gut microbiota in mice fed an HFD. The richness and diversity of gut microbiota were notably decreased by melatonin. HFD feeding altered 69 operational taxonomic units (OTUs) compare with a normal chow diet (NCD) group, and melatonin supplementation reversed 14 OTUs to the same configuration than those present in the NCD group, thereby impacting various functions, in particular through its ability to decrease the Firmicutes-to-Bacteroidetes ratio and increase the abundance of mucin-degrading bacteria Akkermansia, which is associated with healthy mucosa. Taken together, our results suggest that melatonin may be used as a probiotic agent to reverse HFD-induced gut microbiota dysbiosis and help us to gain a better understanding of the mechanisms governing the various melatonin beneficial effects.
Experiment 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity,Obesity
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- normal chow diet
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- high fat diet
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Obesity is (BMI ≥ 30kg/m2)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 5
- Group 1 sample size Number of subjects in the case (exposed) group
- 5
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Figure 6B + Data S1
Description: Differential abundant taxa in gut microbiota in response to melatonin treatment
Abundance in Group 1: increased abundance in high fat diet
| NCBI | Quality Control | Links |
|---|---|---|
| Acetitomaculum | ||
| Prevotellaceae | ||
| Ruminococcus | ||
| Marvinbryantia | ||
| Alistipes | ||
| Blautia |
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Figure 6B + Data S1
Description: Differential abundant taxa in gut microbiota in response to melatonin treatment
Abundance in Group 1: decreased abundance in high fat diet
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides | ||
| Odoribacter | ||
| Anaerovorax | ||
| Butyricimonas | ||
| Christensenellaceae | ||
| Helicobacter | ||
| Christensenella | ||
| Bilophila | ||
| Mycoplasmatota | ||
| Oscillibacter |
Revision editor(s): WikiWorks
Experiment 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- high fat diet
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- high fat diet + melatonin
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Figure 6B + Data S1
Description: Differential abundant taxa in gut microbiota in response to melatonin treatment
Abundance in Group 1: increased abundance in high fat diet + melatonin
| NCBI | Quality Control | Links |
|---|---|---|
| Proteus | ||
| Odoribacter | ||
| Helicobacter | ||
| Anaerovorax | ||
| Desulfovibrio | ||
| Desulfovibrionaceae | ||
| Butyricimonas | ||
| Akkermansia | ||
| Antricoccus |
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Figure 6B + Data S1 + Figure 7
Description: Differential abundant taxa in gut microbiota in response to melatonin treatment
Abundance in Group 1: decreased abundance in high fat diet + melatonin
| NCBI | Quality Control | Links |
|---|---|---|
| Alistipes | ||
| Anaerotruncus | ||
| Helicobacter marmotae |
Revision editor(s): WikiWorks
Retrieved from "https://bugsigdb.org/w/index.php?title=Study_16&oldid=114242"
Hidden category:
