Regulatory T Cells and Plasmacytoid Dendritic Cells Within the Tumor Microenvironment in Gastric Cancer Are Correlated With Gastric Microbiota Dysbiosis: A Preliminary Study

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Reviewed Marked as Reviewed by Atrayees on 2023-7-25
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Ling Z, Shao L, Liu X, Cheng Y, Yan C, Mei Y, Ji F, Liu X
Journal
Frontiers in immunology
Year
2019
Keywords:
Tregs (Regulatory T cells), gastric cancer, microbiota (microorganism), pDCs, tumor microenvironment
Substantial evidence indicates that gastric microbiota dysbiosis, immune system dysfunction especially immune escape are critical for gastric cancer (GC) occurrence and progression. As two important elements of tumor microenvironment (TME), the relationship between gastric microbiota and tumor-immune microenvironment is still unclear. Our present study aimed to explore the correlation between gastric mucosal microbiota in different microhabitats and its corresponding gastric immunosuppressive cells such as regulatory T cells (Tregs) and plasmacytoid dendritic cells (pDCs) in the TME. A cohort of 64 GC patients without preoperative chemotherapy was enrolled retrospectively, and 60 normal, 61 peritumoral and 59 tumoral tissues were obtained for gastric mucosal microbiota analysis and immunohistochemistry analysis. From different microhabitats, BDCA2+pDCs and Foxp3+Tregs were observed positively correlated, and increased in tumoral and peritumoral tissues compared to normal ones. The diversity, composition and function of gastric mucosal microbiota also changed more significantly in tumoral tissues than those in normal and peritumoral ones. With pearson's correlation analysis, we found that several non-abundant genera such as Stenotrophomonas and Selenomonas were positively correlated with BDCA2+pDCs and Foxp3+Tregs, respectively, while Comamonas and Gaiella were negatively correlated with BDCA2+pDCs and Foxp3+ Tregs, respectively. The increased BDCA2+pDCs and Foxp3+Tregs might be modulated by gastric mucosal microbiota, both participated in the immunosuppression microenvironment of GC, which might provide evidence to establish new strategies in antitumor therapy targeting on gastric microbiota.

Experiment 1


Reviewed Marked as Reviewed by Atrayees on 2023-7-25

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Stomach Anterior intestine,Gaster,Mesenteron,Stomach chamber,Ventriculus,Stomach,stomach
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Gastric cancer Ca body - stomach,ca greater curvature of stomach,Ca lesser curvature - stomach,cancer of stomach,gastric cancer,gastric cancer, intestinal,gastric neoplasm,malignant gastric neoplasm,malignant gastric tumor,malignant neoplasm of body of stomach,malignant neoplasm of lesser curve of stomach,malignant neoplasm of stomach,malignant neoplasm of the stomach,malignant stomach neoplasm,malignant tumor of body of stomach,malignant tumor of greater curve of stomach,malignant tumor of lesser curve of stomach,malignant tumor of stomach,malignant tumor of the stomach,stomach cancer,Gastric cancer
Group 0 name Corresponds to the control (unexposed) group for case-control studies
normal
Group 1 name Corresponds to the case (exposed) group for case-control studies
gastric cancer peritumor group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
gastric cancer
Group 0 sample size Number of subjects in the control (unexposed) group
60
Group 1 sample size Number of subjects in the case (exposed) group
61
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
2
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Simpson Estimator of species richness and species evenness: more weight on species evenness
decreased
Richness Number of species
decreased

Signature 1

Reviewed Marked as Reviewed by Atrayees on 2023-7-25

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 3

Description: LEfSe identifies the taxa with the greatest differences in abundance among the three stomach microhabitats

Abundance in Group 1: increased abundance in gastric cancer peritumor group

NCBI Quality ControlLinks
Oceanospirillales
Halomonas
Halomonadaceae
Shewanellaceae
Shewanella
Alteromonadales
Enterococcus
Enterococcaceae
Brevundimonas
Arthrobacter
Mycobacterium
Mycobacteriaceae

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Atrayees on 2023-7-25

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 3

Description: LEfSe identifies the taxa with the greatest differences in abundance among the three stomach microhabitats

Abundance in Group 1: decreased abundance in gastric cancer peritumor group

NCBI Quality ControlLinks
Cupriavidus
Burkholderiaceae
Porphyromonadaceae
Legionellaceae
Legionella
Legionellales

Revision editor(s): WikiWorks

Experiment 2


Reviewed Marked as Reviewed by Atrayees on 2023-7-25

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
peritumor
Group 1 name Corresponds to the case (exposed) group for case-control studies
gastric cancer tumor group
Group 0 sample size Number of subjects in the control (unexposed) group
61
Group 1 sample size Number of subjects in the case (exposed) group
59

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Atrayees on 2023-7-25

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 3

Description: LEfSe identifies the taxa with the greatest differences in abundance among the three stomach microhabitats

Abundance in Group 1: increased abundance in gastric cancer tumor group

NCBI Quality ControlLinks
Bacilli
Enterococcus
Enterococcaceae
Selenomonas
Oceanospirillales
Halomonas
Halomonadaceae
Bifidobacteriaceae
Bifidobacterium
Bifidobacteriales
Enterobacteriaceae
Enterobacterales
Alistipes
Ruminococcus
Rikenellaceae
Dialister
Oscillibacter
Coprococcus
Oscillospiraceae
Roseburia
Megasphaera
Staphylococcaceae
Staphylococcus
Blautia

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Atrayees on 2023-7-25

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 3

Description: LEfSe identifies the taxa with the greatest differences in abundance among the three stomach microhabitats

Abundance in Group 1: decreased abundance in gastric cancer tumor group

NCBI Quality ControlLinks
Rhizobium
Rhizobiaceae
Bacteroidota
Megamonas
Shewanellaceae
Shewanella
Alteromonadales
Mycobacterium
Mycobacteriaceae
Lactobacillaceae
Lactobacillus
Pseudomonadota

Revision editor(s): WikiWorks

Experiment 3


Reviewed Marked as Reviewed by Atrayees on 2023-7-25

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
normal
Group 0 sample size Number of subjects in the control (unexposed) group
60

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
decreased

Signature 1

Reviewed Marked as Reviewed by Atrayees on 2023-7-25

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): Lwaldron, WikiWorks

Source: Figure 3

Description: LEfSe identifies the taxa with the greatest differences in abundance among the three stomach microhabitats

Abundance in Group 1: increased abundance in gastric cancer tumor group

NCBI Quality ControlLinks
Actinomycetota
Alteromonadales
Aquabacterium
Bacilli
Bifidobacteriaceae
Bifidobacteriales
Bifidobacterium
Blautia
Burkholderiales
Carnobacteriaceae
Coprococcus
Dialister
Enterobacterales
Enterobacteriaceae
Bacillota
Gemella
Gemmiger
Granulicatella
Hydrogenophilaceae
Hydrogenophilales
Hydrogenophilus
Lactobacillaceae
Lactobacillales
Lactobacillus
Massilia
Methylobacteriaceae
Methylobacterium
Neisseria
Neisseriaceae
Neisseriales
Oscillospiraceae
Oxalobacteraceae
Peptostreptococcaceae
Peptostreptococcus
Propionibacteriaceae
Propionibacterium
Roseburia
Rubrobacter
Rubrobacteraceae
Rubrobacterales
Ruminococcus
Shewanella
Shewanellaceae
Streptococcaceae
Streptococcus
Veillonella
Veillonellaceae

Revision editor(s): Lwaldron, WikiWorks

Signature 2

Reviewed Marked as Reviewed by Atrayees on 2023-7-25

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 3

Description: LEfSe identifies the taxa with the greatest differences in abundance among the three stomach microhabitats

Abundance in Group 1: decreased abundance in gastric cancer tumor group

NCBI Quality ControlLinks
Bacteroidota
Porphyromonadaceae
Corynebacterium
Corynebacteriaceae
Burkholderiaceae
Cupriavidus
Actinomycetaceae
Actinomyces
Staphylococcaceae
Staphylococcus

Revision editor(s): WikiWorks