High-fat feeding rather than obesity drives taxonomical and functional changes in the gut microbiota in mice/Experiment 3

From BugSigDB


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Subjects

Location of subjects
Denmark
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity,Obesity
Group 0 name Corresponds to the control (unexposed) group for case-control studies
BL6-High Fat with enomithacin
Group 1 name Corresponds to the case (exposed) group for case-control studies
Sv129-High Fat with enomithacin
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
(BL6): C57BL/6JBomTac mouse strain, (Sv129): the obesity-resistant mouse strain 129S6/SvEvTac . LF: (LF)low-fat diet, (HF) high fat diet without indomethacin, (HFI) high fat diet supplemented with indomethacin.
Group 0 sample size Number of subjects in the control (unexposed) group
10
Group 1 sample size Number of subjects in the case (exposed) group
9

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

Revision editor(s): WikiWorks

Source: Data S4,S7,S8,S9,S10,S11,S13,S14

Description: Relative abundance at the bacterial phylum, genus and species levesl between two strain mices fed low fat diet, fed high fat diet and high fat diet + indomethacin

Abundance in Group 1: increased abundance in Sv129-High Fat with enomithacin

NCBI Quality ControlLinks
Bacteroides
Bacteroidota
Cyanobacteriota
Chlorobiota
Fibrobacterota

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Fatima on 2021/07/28

Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

Revision editor(s): Fatima, Kwekuamoo, WikiWorks

Source: Data S4,S7,S8,S9,S10,S11,S13,S14

Description: Relative abundance at the bacterial phylum, genus and species levesl between two strain mices fed low fat diet, fed high fat diet and high fat diet + indomethacin

Abundance in Group 1: decreased abundance in Sv129-High Fat with enomithacin

NCBI Quality ControlLinks
Actinomycetota
Akkermansia
Akkermansia muciniphila
Anaerotruncus
[Clostridium] leptum CAG:27
Fusobacteriota
Holdemanella biformis
Planctomycetota
Verrucomicrobiota
Peptacetobacter hiranonis

Revision editor(s): Fatima, Kwekuamoo, WikiWorks