Altered Gut Microbiota in Irritable Bowel Syndrome and Its Association with Food Components

Study information

Reviewed Marked as Reviewed by Aiyshaaaa on 2023-6-3

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

33429936

DOI Digital object identifier for electronic documents.

https://doi.org/10.3390/jpm11010035

URI

Authors

Barandouzi ZA, Lee J, Maas K, Starkweather AR, Cong XS

Journal

Journal of personalized medicine

Year

2021

Keywords:

food components, irritable bowel syndrome, microbiome, microbiota, nutrients

The interplay between diet and gut microbiota has gained interest as a potential contributor in pathophysiology of irritable bowel syndrome (IBS). The purpose of this study was to compare food components and gut microbiota patterns between IBS patients and healthy controls (HC) as well as to explore the associations of food components and microbiota profiles. A cross-sectional study was conducted with 80 young adults with IBS and 21 HC recruited. The food frequency questionnaire was used to measure food components. Fecal samples were collected and profiled by 16S rRNA Illumina sequencing. Food components were similar in both IBS and HC groups, except in caffeine consumption. Higher alpha diversity indices and altered gut microbiota were observed in IBS compared to the HC. A negative correlation existed between total observed species and caffeine intake in the HC, and a positive correlation between alpha diversity indices and dietary fiber in the IBS group. Higher alpha diversity and gut microbiota alteration were found in IBS people who consumed caffeine more than 400 mg/d. Moreover, high microbial diversity and alteration of gut microbiota composition in IBS people with high caffeine consumption may be a clue toward the effects of caffeine on the gut microbiome pattern, which warrants further study.

Experiment 1

Edit History Delete

Flag for review

Your review change was submittedDuplicate this Experiment Add a new Signature

Reviewed Marked as Reviewed by Aiyshaaaa on 2023-6-3

Curated date: 2022/10/25

Curator: Fatima

Revision editor(s): Fatima, Aiyshaaaa

Subjects

Location of subjects: Canada

Host species Species from which microbiome was sampled (if applicable): Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: irritable bowel syndrome [X]Psychogenic IBS,Adaptive colitis,Colitides, Mucous,Colitis, Mucous,Colon spasm,Colon, Irritable,Functional bowel disease,IBD,IBS,IBS - Irritable bowel syndrome,IC - Irritable colon,Irritable bowel,Irritable bowel - IBS,irritable bowel syndrome,Irritable Bowel Syndromes,Irritable Colon,irritable colon,Irritable colon (disorder),Irritable colon - Irritable bowel syndrome,Irritable colon syndrome,Membranous colitis,Mucous Colitides,Mucous colitis,mucus colitis,Nervous colitis,Psychogenic IBS,psychogenic IBS,Spastic colitis,Spastic colon,spastic colon,Syndrome, Irritable Bowel,Syndromes, Irritable Bowel

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy control
Group 1 name Corresponds to the case (exposed) group for case-control studies: IBS
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: The patient's who have been diagnosed from irritable bowel syndrome(IBS).
Group 0 sample size Number of subjects in the control (unexposed) group: 21
Group 1 sample size Number of subjects in the case (exposed) group: 80
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): current

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: increased
Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Signature 1

Edit History Delete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by Aiyshaaaa on 2023-6-3

Curated date: 2022/10/25

Curator: Fatima

Revision editor(s): Fatima, Aiyshaaaa

Source: Figure 2(b)

Description: Taxonomic differences of fecal microbiota between IBS and HC groups

Abundance in Group 1: increased abundance in IBS

NCBI	Quality Control	Links
Akkermansia
Bacilli
Blautia
Coriobacteriaceae
Coriobacteriales
Coriobacteriia
Erysipelotrichaceae
Erysipelotrichales
Erysipelotrichia
Flavonifractor
Lachnospiraceae
Lactobacillales
Odoribacter
Oscillibacter
Parabacteroides
Porphyromonadaceae
Veillonella
Verrucomicrobiales
Verrucomicrobiota
unclassified Lachnospiraceae
Verrucomicrobiae