Altered Gut Microbiota in Irritable Bowel Syndrome and Its Association with Food Components

Study information

Needs review

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

33429936

DOI Digital object identifier for electronic documents.

10.3390/jpm11010035

URI Uniform resource identifier for web resources.

Authors

Barandouzi ZA, Lee J, Maas K, Starkweather AR, Cong XS

Journal

Journal of personalized medicine

Year

2021

The interplay between diet and gut microbiota has gained interest as a potential contributor in pathophysiology of irritable bowel syndrome (IBS). The purpose of this study was to compare food components and gut microbiota patterns between IBS patients and healthy controls (HC) as well as to explore the associations of food components and microbiota profiles. A cross-sectional study was conducted with 80 young adults with IBS and 21 HC recruited. The food frequency questionnaire was used to measure food components. Fecal samples were collected and profiled by 16S rRNA Illumina sequencing. Food components were similar in both IBS and HC groups, except in caffeine consumption. Higher alpha diversity indices and altered gut microbiota were observed in IBS compared to the HC. A negative correlation existed between total observed species and caffeine intake in the HC, and a positive correlation between alpha diversity indices and dietary fiber in the IBS group. Higher alpha diversity and gut microbiota alteration were found in IBS people who consumed caffeine more than 400 mg/d. Moreover, high microbial diversity and alteration of gut microbiota composition in IBS people with high caffeine consumption may be a clue toward the effects of caffeine on the gut microbiome pattern, which warrants further study.

Experiment 1

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incomplete

Curated date: 2022/10/25

Curator: Fatima

Revision editor(s): Fatima

Subjects

Location of subjects: Canada

Host species Species from which microbiome was sampled (if applicable): Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy control
Group 1 name Corresponds to the case (exposed) group for case-control studies: IBS
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: diagnosis of IBS from a healthcare provider using the Rome III or IV criteria
Group 0 sample size Number of subjects in the control (unexposed) group: 21
Group 1 sample size Number of subjects in the case (exposed) group: 80
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): current

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: increased
Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Signature 1

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Curated date: 2022/10/25

Curator: Fatima

Revision editor(s): Fatima

Source: Figure 2

Description: Taxonomic differences of fecal microbiota between IBS and HC groups

Abundance in Group 1: increased abundance in IBS

NCBI	Links
Lactobacillales
Bacilli
Coriobacteriales
Coriobacteriia
Coriobacteriaceae
Flavonifractor
Erysipelotrichia
Erysipelotrichales
Erysipelotrichaceae
Odoribacter
Veillonella
Verrucomicrobiota
Verrucomicrobiales
Oscillibacter
Blautia
unclassified Lachnospiraceae
Akkermansia
Parabacteroides
Porphyromonadaceae
Lachnospiraceae