Environmental toxicants in breast milk of Norwegian mothers and gut bacteria composition and metabolites in their infants at 1 month/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-25
Subjects
- Location of subjects
- Norway
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Environmental exposure measurement Environmental exposure measurement,environmental exposure measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- one-month age infants exposed to low chemical breast milk (<20th percentile)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- one-month age infants exposed to high chemical breast milk
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- infants with high chemical exposure (≥ 80th percentile breast milk toxicant exposure)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 45
- Group 1 sample size Number of subjects in the case (exposed) group
- 45
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 2 weeks
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- gestational age
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-25
Curated date: 2021/01/10
Curator: Shaimaa Elsafoury
Revision editor(s): Fatima, LGeistlinger, WikiWorks
Source: Figure 2
Description: Differentially abundant sequences in the high vs. low chemical exposure groups.
Abundance in Group 1: increased abundance in one-month age infants exposed to high chemical breast milk
| NCBI | Quality Control | Links |
|---|---|---|
| Clostridium | ||
| Enterococcus | ||
| Streptococcaceae |
Revision editor(s): Fatima, LGeistlinger, WikiWorks
Signature 2
Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-25
Source: Figure 2
Description: Differentially abundant sequences in the high vs. low chemical exposure groups.
Abundance in Group 1: decreased abundance in one-month age infants exposed to high chemical breast milk
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillus | ||
| Veillonella |
Revision editor(s): WikiWorks
